| 姜冉冉,江润海,朱城强,侯秀丽.EDTA对铅胁迫下狗牙根根际土壤质量及微生物的影响[J].农业环境科学学报,2022,41(12):2722-2732. |
| EDTA对铅胁迫下狗牙根根际土壤质量及微生物的影响 |
| Effects of EDTA on soil quality and microorganisms in rhizosphere of Cynodon dactylon under lead stress |
| 投稿时间：2022-11-07 修订日期：2022-12-01 |
| DOI：10.11654/jaes.2022-1123 |
| 中文关键词: 狗牙根 乙二胺四乙酸（EDTA） 铅 高通量测序 |
| 英文关键词: Cynodon dactylon EDTA lead high-throughput sequencing |
| 基金项目:国家自然科学基金地区基金项目（42167009）；云南省高校联合基金项目（2018FH001-004）；昆明学院农业资源利用科技创新团队项目 |
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| 中文摘要: |
| 为研究螯合剂乙二胺四乙酸(EDTA)对狗牙根根际土壤理化性质、土壤酶及微生物群落结构的影响,本研究以耐性植物狗牙根(Cynodon dactylon)为研究对象,在铅污染土壤中施加不同浓度的EDTA[0(对照)、2.5、5.0、7.5、10.0mmol·L-1]进行盆栽实验。结果表明:施加2.5mmol·L-1EDTA时,狗牙根根际土壤pH最低,为6.80,土壤有效磷含量、土壤酸性磷酸酶活性、蔗糖酶活性最高,分别是对照组的2.24、1.57、1.41倍;施加7.5mmol·L-1EDTA时,根际土壤有效钾、铵态氮、硝态氮含量最高,分别为96.22、262.00、15.90mg·kg-1,分别是对照组的1.09、1.30、6.41倍。施加7.5mmol·L-1EDTA时,狗牙根根际土壤微生物多样性以及OTU数量最高,且绿弯菌门(Chloroflexi)、嗜酸杆菌属(Acidibacter)、土微菌属(Pedomicrobium)、曲霉属(Aspergillus)相对丰度较对照组分别增加了7、3、10、8个百分点。施加EDTA后,狗牙根根际土壤中芽孢杆菌属(Bacillus)最高相对丰度较对照组增加了9个百分点,枝孢属(Cladosporium)最高相对丰度是对照组的4.05倍。土壤酸性磷酸酶、脲酶、蔗糖酶活性和有效磷含量均与曲霉属、Gibellulopsis呈正相关,同时土壤脲酶活性与耐重金属污染的菌群[芽孢杆菌属、毛壳菌属(Chaetomium)]呈正相关,表明耐重金属铅污染的微生物有助于提高土壤有效氮和有效磷含量。研究表明,在铅污染土壤中,施加适量浓度的EDTA有助于提高植物根际土壤中酸性磷酸酶和蔗糖酶活性,提高微生物多样性及丰富度,改善根际土壤微生态环境,提高耐性植物对重金属的修复效果。 |
| 英文摘要: |
| To study the effects of the chelating agent ethylenediamine tetraacetic acid（EDTA）on the physicochemical properties, soil enzymes, and microbial community structure in rhizosphere soil of Cynodon dactylon, different concentrations of EDTA[0（CK）, 2.5, 5.0, 7.5, 10.0 mmol·L-1] were applied on lead-contaminated soil, in which the tolerant plant Cynodon dactylon was planted in a pot experiment. The results were as follows：When 2.5 mmol·L-1 EDTA was applied to the rhizosphere soil of Cynodon dactylon, the lowest soil pH was 6.80, and the activities of soil available phosphorus（AP）, soil acid phosphatase, and invertase were the highest, and they were 2.24, 1.57 times, and 1.41 times that of those of the CK, respectively; When 7.5 mmol·L-1 EDTA was applied, the content of available potassium（AK）, ammonium nitrogen, and nitrate nitrogen in rhizosphere soil was the highest, and their values（96.22, 262.00 mg·kg-1, and 15.90 mg·kg-1） were 1.09, 1.30 times, and 6.41 times that of those of the CK, respectively. The diversity of soil microorganisms in the rhizosphere soil of Cynodon dactylon and the number of OTU in the EDTA concentration of 7.5 mmol · L-1 were the highest. Chloroflexi, Acidibacter, Pedomicrobium, and Aspergillus, with the highest relative abundance, increased by 7, 3, 10, 8 percentage points over the CK, respectively. After applying EDTA, the highest relative abundance of Bacillus in the rhizosphere soil of Cynodon dactylon increased by 9 percentage points over the CK; The highest relative abundance of Cladosporium was 4.05 times that of the CK. The activities of soil acid phosphatase, urease, sucrase, and AP positively correlated with Aspergillus and Gibellulopsis, whereas soil urease positively correlated with heavy metalresistant bacteria（Bacillus and Chaetomium）, indicating that heavy metal-resistant microorganisms contributed to the increased content of available nitrogen and phosphorus in the soil. In lead-contaminated soil, the application of appropriate concentration of EDTA can increase the acid phosphatase, invertase activities of plant rhizosphere soil, and diversity and abundance of microorganisms; improves the microecological environment of rhizosphere soil; then improves the remediation effect of tolerant plants on heavy metals. |
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