文章摘要
李运朝,李洪涛,及华,李俊花,李超楠,章丽,金小艳.不同生物制剂对南方根结线虫杀虫活性与防治效果[J].农业环境科学学报,2022,41(12):2810-2816.
不同生物制剂对南方根结线虫杀虫活性与防治效果
Insecticidal activity and control effect of different biological preparations against Meloidogyne incognita
投稿时间:2022-11-10  修订日期:2022-12-13
DOI:10.11654/jaes.2022-1146
中文关键词: 番茄根结线虫病  南方根结线虫  生物防治  芽孢杆菌  木醋液
英文关键词: tomato root-knot nematode disease  Meloidogyne incognita  biological control  Bacillus  wood vinegar
基金项目:河北省重点研发计划项目(20326505D,20326421D)
作者单位E-mail
李运朝 河北省农林科学院生物技术与食品科学研究所, 石家庄 050051  
李洪涛 河北省农林科学院生物技术与食品科学研究所, 石家庄 050051 lihongtao1314@yeah.net 
及华 河北省农林科学院生物技术与食品科学研究所, 石家庄 050051  
李俊花 河北省农林科学院生物技术与食品科学研究所, 石家庄 050051  
李超楠 河北省农林科学院生物技术与食品科学研究所, 石家庄 050051
河北农业大学植物保护学院, 河北 保定 071000 
 
章丽 河北省农林科学院生物技术与食品科学研究所, 石家庄 050051  
金小艳 中农绿康生物技术有限公司, 北京 102101  
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中文摘要:
      为探讨不同生物制剂对南方根结线虫杀虫活性及其防治效果,采用实验室分离培养的10株芽孢杆菌和2种山杏壳木醋液对南方根结线虫进行杀虫活性筛选,并利用温室盆栽番茄实验和番茄田间试验对筛选出的制剂进行防治效果验证。室内生测验证实验表明,有4株芽孢杆菌发酵液和山杏壳木醋液对南方根结线虫有较好的毒杀作用。菌株XYG169发酵液对线虫校正死亡率为84.9%;菌株BMT295发酵液对线虫校正死亡率为100%;菌株BMT256发酵液对线虫校正死亡率为97.9%;菌株YLT74发酵液对线虫校正死亡率为80.7%。山杏壳木醋液稀释100、200、400、600倍和800倍溶液的线虫校正死亡率分别为100%、100%、92.8%、65.4%和42.5%。盆栽实验表明BMT295粉剂4g+山杏壳木醋液300倍液处理防效最好,相对防效为74.00%。田间试验表明BMT295粉剂+山杏壳木醋液300倍液处理对南方根结线虫防治效果最好,其相对防效为64.54%,该结论与盆栽实验结论一致。综合室内生测实验、盆栽实验和田间试验结果,BMT295粉剂和山杏壳木醋液联合使用防治效果显著,可以作为生物杀线虫剂进一步在田间试验推广。
英文摘要:
      This study explores the insecticidal activity and control effect of different biological agents on Meloidogyne incognita. Ten Bacillus species and two kinds of Prunus armeniaca(apricot)shell wood vinegar were isolated and cultured in our laboratory and were tested for their insecticidal activity screening against Meloidogyne incognita. The control efficacy of the selected agents was verified using greenhouse pot-grown and field-grown tomatoes as testing plants. The indoor bioassay verification test showed that the fermentation broths of four Bacillus strains and the wild P. armeniaca shell wood vinegar had good poisoning effect on Meloidogyne incognita. The corrected mortalities for the Bacillus fermentation broths were 84.9% for strain XYG169, 100% for BMT295, 97.9% for strain BMT256, and 80.7% for the YLT74. The corrected mortality rates of nematodes with 100-fold, 200-fold, 400-fold, 600-fold and 800-fold diluted solutions of P. armeniaca shell wood vinegar were 100%, 100%, 92.8%, 65.4%, and 42.5%, respectively. The pot experiment in the greenhouse showed that the control effect of the 300-fold solution of P. armeniaca wood vinegar added with 4 g of BMT295 powder was the most effective, with a relative control effect of 74.00%. The field experiment showed that the same treatment of 300-fold solution had the best control effect on Meloidogyne incognita, with a relative control effect of 64.54%. The results of indoor bioassay and pot and field experiment revealed that the BMT295 powder mixed with P. armeniaca wood vinegar had a noticeable control effect, which could be used as a biological nematicide for further field experiment and popularization.
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