| 李超楠,李洪涛,李运朝,李俊花,及华,章丽,王琳.北苍术根腐病病原菌分离鉴定及其生防菌筛选[J].农业环境科学学报,2022,41(12):2824-2830. |
| 北苍术根腐病病原菌分离鉴定及其生防菌筛选 |
| Isolation and identification of root rot pathogen of Atractylodes chinensis and screening of biocontrol bacteria |
| 投稿时间：2022-11-08 修订日期：2022-11-29 |
| DOI：10.11654/jaes.2022-1131 |
| 中文关键词: 北苍术 根腐病 尖孢镰刀菌 生防芽孢杆菌 药用植物 |
| 英文关键词: Atractylodes chinensis root rot Fusarium oxysporum biocontrol bacillus medicinal plant |
| 基金项目:河北省重点研发计划项目（20326505D，20326421D） |
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| 中文摘要: |
| 为明确导致北苍术根腐病的病原菌，本实验从承德市感病北苍术块茎中分离病原微生物，并对病原微生物致病性进行回接验证。研究分离获得的具有致病性的病原菌鉴定为尖孢镰刀菌（Fusarium oxysporum）。从健康药用植物根际土壤中分离获得94株根际微生物，通过平板拮抗筛选获得2株生防菌，分别为菌株SFJ-27和菌株SFJ-41，平板对峙实验结果表明2株生防菌抑菌率均在50%以上。通过分子生物学和形态学鉴定2株菌分别为枯草芽孢杆菌（Bacillus subtilis）和贝莱斯芽孢杆菌（Bacillusvelezensis）。盆栽实验结果表明，菌株SFJ-27与菌株SFJ-41对北苍术根腐病具有明显的生防效果，防治效果分别为52.27%和68.19%。研究表明，尖孢镰刀菌（Fusarium oxysporum）可以导致北苍术根腐病的发生，枯草芽孢杆菌（Bacillus subtilis）和贝莱斯芽孢杆菌（Bacillus velezensis）对北苍术根腐病具有防治作用。 |
| 英文摘要: |
| To identify the pathogen of root rot of Atractylodes chinensis, pathogenic microorganisms were isolated from the tuber of Atractylodes chinensis in Chengde, and the pathogenicity of the pathogenic microorganisms was verified via back-grafting. The isolated pathogenic bacterium with pathogenicity was identified as Fusarium oxysporum. A total of 94 rhizosphere microorganisms were isolated from the rhizosphere soil of healthy medicinal plants, and two biocontrol strains, strain SFJ-27 and strain SFJ-41, were obtained through plate antagonism screening. Plate antagonism test results showed that the inhibition rates of the two biocontrol strains were above 50%. It was identified as Bacillus subtilis and Bacillus velezensis according to molecular biology and morphology. Pot experiments showed that strain SFJ-27 and strain SFJ-41 had significant biocontrol effects on root rot of Atractylodes chinensis, with control effects of 52.27% and 68.19%, respectively. Studies have shown that Fusarium oxysporum can cause root rot of Atractylodes chinensis and Bacillus subtilis and Bacillus velezensis have preventive effect on the root rot of Atractylodes chinensis. |
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