In order to study the feasibility of lychee pruning compost and vermicompost to replace turf as carrier of Priestia megaterium. In present experiment, we comprised six mixed carriers to the target bacteria of Priestia megaterium by using different ratio of lychee pruning compost, vermicompost and turf, and ranked as ST1, ST2, ST3, ST4, ST5, ST6 with mass ratio of 6:2:2, 4:2:4, 2:2:6, 6:3:1, 4:3:3, 2:3:5. The turf (CK) was used as control. We dynamically monitored the viable counts in carriers at different cultivation time, and then obtaining the mixed carrier formula suitable for the survival of Priestia megaterium. On this basis, we set water content (20%, 30%, 40%), temperature (20, 30, 40, 50 ℃) and inoculation concentration (106, 107, 108 cfu·mL-1), and dynamically monitored the living bacteria, and then optimize the water content, temperature and inoculation concentration of carriers. The results showed that viable count of each carrier decreased first and then increased with the cultivation time. ST2 and ST5 both were the optimal carrier of Priestia megaterium for the high viable counts after long term of cultivation and low turf addition. After 60 days of cultivation, with the increase of water content and temperature of the carrier, viable count increased first and then decreased, and reached the highest value at 30% water content (ST2: 2.46×108 cfu·g-1, ST5: 1.81×108 cfu·g-1) and 30 ℃ (ST2: 3.44×108 cfu·g-1, ST5: 1.87×108 cfu·g-1) and 40 ℃ (ST2: 8.50×107 cfu·g-1, ST5: 7.13×107 cfu·g-1) respectively. Moreover, the viable count in carrier at each cultivation stage increased with inoculation concentrations, the viable counts of ST2 and ST5 reached 3.63×108, 3.33×108 cfu·g-1 respectively after 60 days of cultivation. The mixed carriers ST2 and ST5 were most suitable to replace turf as the carrier of Priestia megaterium, and the optimal conditions for the carriers were 30 % water content, 30~40 ℃ temperature, and 108 cfu·mL-1 inoculation concentration. This study provided technical support for turf saving and the utilization of agricultural wastes. |