| 古鹏,汤佳豪,赵庆庆,王磊磊,王加宁,卢媛,张闻.土壤中9-芴酮和9，10-蒽醌的分析方法及赋存形态[J].农业环境科学学报,2023,42(5):1091-1099. |
| 土壤中9-芴酮和9，10-蒽醌的分析方法及赋存形态 |
| Analytical method and speciation characteristics of 9-fluorenone and 9, 10-anthraquinone in soil |
| 投稿时间：2022-12-27 |
| DOI：10.11654/jaes.2022-1301 |
| 中文关键词: 含氧多环芳烃 土壤 赋存形态 生物有效性 9-芴酮 9,10-蒽醌 |
| 英文关键词: oxygenated polycyclic aromatic hydrocarbons(OPAHs) soil speciation bioavailability 9-fluorenone 9, 10-anthraquinone |
| 基金项目:山东省自然科学基金项目（ZR2022MD116）；国家重点研发计划项目（2019YFC1804103）；山东省重点研发计划项目（2021CXGC011201） |
| 作者 | 单位 | E-mail | | 古鹏 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 汤佳豪 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 赵庆庆 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 王磊磊 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 王加宁 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 卢媛 | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津 300350 | | | 张闻 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | zw-sunshine@163.com |
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| 中文摘要: |
| 为探究含氧多环芳烃（oxygenated polycyclic aromatic hydrocarbon，OPAHs）在土壤中的赋存形态特征，以9-芴酮和9，10-蒽醌为OPAHs模式物，建立了其在不同基质中的提取及测定方法，分析了其在土壤中30 d内的赋存形态。结果表明：以甲醇-二氯甲烷（1∶1，V/V）溶液为萃取剂，采用超声-高效液相色谱法提取测定土壤中的9-芴酮和9，10-蒽醌，回收率为102.4%~104.2%。以二氯甲烷为萃取剂，通过液液萃取-高效液相色谱法测定水相及生物有效态相（羟丙基-β-环糊精）中的9-芴酮和9，10-蒽醌，回收率分别为87.2%~87.8%和78.7%~86.3%。采用连续提取方法，研究了这2种OPAHs在土壤中30 d内的形态分布，各形态9-芴酮和9，10-蒽醌含量之和的顺序为难解吸态>粗胡敏素结合态>干酪根结合态>生物有效态>胡敏酸结合态>富里酸结合态>矿物结合态。可提取态（生物有效态与难解吸态加和）为主要存在形态（58.0%），结合态亦占有相当大的比例（42.0%），有机质结合态较矿物结合态含量占比更高。土壤中9-芴酮和9，10-蒽醌在前15 d生物有效性降低较快，后15 d趋缓，9-芴酮较9，10-蒽醌生物有效性下降更快。 |
| 英文摘要: |
| To explore the speciation characteristics of typical oxygenated polycyclic aromatic hydrocarbon(OPAHs) in soil, 9-fluorenone and 9, 10-anthraquinone were used as representative OPAHs and their extraction and determination methods in different environmental media were established. Their various speciations in soil within 30 d were analyzed. The results showed that the recovery of 9-fluorenone and 9, 10-anthraquinone in soil was 102.4%-104.2% using a methanol-dichloromethane(1:1, V/V) solution as an extractant to perform ultrasonic extraction and high performance liquid chromatography(HPLC) analysis. 9-fluorenone and 9, 10-anthraquinone in aqueous and bioavailable(hydroxypropyl-β-cyclodextrin) phases were analyzed by liquid-liquid extraction with dichloromethane as an extractant followed by HPLC, and the recoveries were 87.2%-87.8% and 78.7%-86.3%, respectively. The speciation distribution of the two OPAHs in soil within 30 d was studied using the successive extraction method. The order of the sum of the contents of 9-fluorenone and 9, 10-anthraquinone in different forms was non-desorbed form > crude humin-bound form > kerogen-bound form > bioavailable form > humic acid-bound form > fulvic acid-bound form > mineral-bound form. The extractable form, which is the sum of the bioavailable and nondesorbed forms, was the main component(58.0%), and the bound form also accounted for a large proportion(42.0%). The content of OPAHs bound to organic matter was higher than that bound to minerals. The bioavailability of 9-fluorenone and 9, 10-anthraquinone decreased rapidly within the first 15 d and slowed down during the subsequent 15 d. The bioavailability of 9-fluorenone decreased faster than that of 9, 10-anthraquinone. |
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