| 伊英达,陈恒雷,冯光文.古菌群落视角评估某退役铀尾矿库的治理效果[J].农业环境科学学报,2023,42(6):1329-1337. |
| 古菌群落视角评估某退役铀尾矿库的治理效果 |
| Evaluation of the remedial effect of a decommissioned uranium tailings pond from the perspective of the archaea community |
| 投稿时间：2022-09-30 修订日期：2022-12-15 |
| DOI：10.11654/jaes.2022-0973 |
| 中文关键词: 退役 铀尾矿库 古菌群落 微生态 治理效果 |
| 英文关键词: decommission uranium tailings pond archaeal community microecology remedial effect |
| 基金项目:国家自然科学基金项目（32060292） |
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| 中文摘要: |
| 为了尝试从微生态－古菌群落视角，评估中国西北某铀尾矿库退役治理效果，本研究在前期尾矿库表层覆土0~5 cm和5~15 cm两个垂向剖面放射性核素、重金属/类金属分布特征研究的基础上，通过16S rRNA基因扩增子测序技术对土壤古菌群落进行测序分析，采用生物信息学方法研究铀尾矿库及毗邻区域土壤古菌群落特征及其对环境因子的响应。结果表明：在尾矿库与毗邻区域，尽管土壤中4个优势菌门和3个优势属呈现不均匀分布，但尾矿库内外土壤古菌优势群落结构基本已无显著差异；土壤古菌群落的α多样性（Chao1、ACE和Shannon指数）和β多样性（PCoA）分析显示群落间已不存在差异，群落组成已具有相似性；冗余分析结果表明，土壤pH、TN（总氮）、TOC（总有机碳）、232Th、EC（电导率）是影响优势菌群的主要环境因子，但并未造成库区内外土壤菌群结构的显著改变。尾矿库与毗邻区域的土壤古菌群落已无明显差异，说明尾矿库内外土壤古菌群落并未受到放射性核素、重金属/类金属的显著影响，反映出尾矿库退役治理效果良好。 |
| 英文摘要: |
| This study aimed to evaluate the effect of retirement management on a uranium tailings pond in northwest China from the perspective of microecological archaeal community. Initially, the early-stage distribution characteristics of radionuclides and heavy metals/ metalloids in the 0-5 cm and 5-15 cm vertical profiles of the overburden layer of the tailings pond were measured. Then, 16S rRNA gene amplification sequencing technology was used to sequence and analyze the soil archaea community, and bioinformatics was used to study the characteristics of the soil archaea community and its response to environmental factors in the uranium tailings pond and adjacent areas. The results demonstrated that there was no significant difference in the structure of soil archaea dominant community between the tailings pond and the adjacent area, even though four dominant phyla and three dominant genera were unevenly distributed in the soil. The diversity indexes（Chao1, ACE, and Shannon index）and similarity analysis（PCoA）of soil archaea showed that there were no differences among the communities, and the community composition was similar. The results of redundancy analysis（RDA）showed that pH, total nitrogen, total organic carbon, 232Th levels, and electrical conductivity of soil were the main environmental factors affecting the dominant flora. However, it did not significantly change the structure of the soil flora inside and outside the tailings pond area. There were no obvious differences between the soil community of the tailings pond and the adjacent area, indicating that the soil community inside and outside the tailings pond were not significantly affected by radionuclides and heavy metals / class metals. These findings reflect that decommissioning the tailings pond was an effective management decision. |
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