| 吴梅,赵家宇,刘怡,田兴磊,倪润祥,刘永林.土壤中砷和硒生物有效态的联合提取[J].农业环境科学学报,2023,42(6):1414-1422. |
| 土壤中砷和硒生物有效态的联合提取 |
| Combined extraction of bioavailability of arsenic and selenium in soils |
| 投稿时间：2022-10-05 修订日期：2022-12-16 |
| DOI：10.11654/jaes.2022-0981 |
| 中文关键词: 土壤 砷 硒 生物有效性 联合提取 |
| 英文关键词: soil arsenic selenium bioavailability combined extraction |
| 基金项目:国家自然科学基金项目（41502329）；重庆市教育委员会科学技术计划项目（KJZDK202100504）；重庆市研究生科研创新项目（CYS22560） |
| 作者 | 单位 | E-mail | | 吴梅 | 重庆师范大学 GIS应用研究重点实验室, 重庆 401331
重庆师范大学地理与旅游学院, 重庆 401331 | | | 赵家宇 | 重庆师范大学 GIS应用研究重点实验室, 重庆 401331
重庆师范大学地理与旅游学院, 重庆 401331 | | | 刘怡 | 重庆师范大学 GIS应用研究重点实验室, 重庆 401331
重庆师范大学地理与旅游学院, 重庆 401331 | | | 田兴磊 | 山东省地质科学研究院, 济南 250013 | | | 倪润祥 | 农业农村部农业生态与资源保护总站, 北京 100125 | | | 刘永林 | 重庆师范大学 GIS应用研究重点实验室, 重庆 401331
重庆师范大学地理与旅游学院, 重庆 401331 | liu3986130@163.com |
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| 中文摘要: |
| 为了快速、准确和有效地联合提取土壤中生物有效态砷和硒，以减少实验成本和提高实验效率，本研究在长期实验摸索及前人工作基础上，研究0.5 mol·L-1 NaHCO3、0.1 mol·L-1 HCl、0.5 mol·L-1 KH2PO4和0.1 mol·L-1 KH2PO4-K2HPO4 4种浸提剂对土壤中生物有效态砷和硒的联合提取实验效果。4种浸提剂所得浸提液经HNO3和HClO4消煮，用氢化物发生原子荧光光度计（HGAFS）测定浸提液中砷与硒的浓度。土壤总砷和总硒的测定中使用两组土壤标准物质控制，玉米标准物质控制玉米根和玉米籽实验质量，相对标准偏差均小于10%，硒和砷的检测限分别为0.01 μg·L-1和0.05 μg·L-1。结果表明： 0.1 mol·L-1 KH2PO4-K2HPO4和0.5 mol·L-1 NaHCO3提取的土壤生物有效态砷、硒含量与土壤总砷（r=0.788，P<0.01； r=0.794，P<0.01）和总硒（r=0.707，P<0.05； r=0.648，P<0.05）均呈显著性正相关，其余两种浸提剂提取效果较差。植物指标法显示，仅0.1 mol·L-1 KH2PO4-K2HPO4提取的土壤生物有效态砷、硒含量与玉米根砷（r=0.848，P<0.01）和硒（r=0.822，P<0.01）呈极显著正相关。研究表明，0.1 mol·L-1 KH2PO4-K2HPO4作为浸提剂可快速、准确、有效地联合提取土壤中砷和硒的生物有效态。 |
| 英文摘要: |
| In this study, to improve experimental efficiency by reducing the number of experimental steps, a rapid, accurate, and effective method for extracting bioavailable As and Se in soils was designed, produced, and evaluated. The experimental effects of four extractants （0.5 mol·L-1 NaHCO3, 0.1 mol·L-1 HCl, 0.5 mol·L-1 KH2PO4 and 0.1 mol·L-1 KH2PO4-K2HPO4）in extracting bioavailable As and Se in soils were compared. Following the mixture and extraction process, the resultant solutions were added to a mixed acid solution of HNO3 and HClO4 and further digested. Subsequently, a hydride generation atomic fluorescence spectrometer（HG-AFS）was used to determine the content of As and Se. Additionally, soil reference materials were used as controls for determining total As and total Se in soils. The experimental quality of corn root and corn seed was controlled using the corn reference material. The relative standard deviation was within 10%, and the detection limits for Se and As were 0.01 μg·L-1and 0.05 μg·L-1, respectively. We found that the contents of soil bioavailable As and Se extracted using 0.1 mol·L-1 KH2PO4-K2HPO4 and 0.5 mol·L-1 NaHCO3 buffer solutions were positively correlated with the total As（r=0.788, P<0.01; r=0.794, P<0.01）and total Se（r=0.707, P<0.05; r=0.648, P<0.05）in soils, whereas the other two extracts were ineffective. The plant index method showed that the content of soil bioavailable As and Se extracted using 0.1 mol·L-1 KH2PO4-K2HPO4 buffer solution had a significant positive relationship only with that of corn root（r=0.848 for As, P<0.01 and r=0.822 for Se, P<0.01）. In conclusion, 0.1 mol·L-1 KH2PO4-K2HPO4 buffer solution could be used as an extractant to rapidly, accurately, and effectively extract bioavailable As and Se in soils. |
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