| 黄鹏,张静,王鸿辉,井江心,曾燕琼,陈铮,刘洋之.腐植酸负载纳米零价铁对镉污染农田水稻籽粒代谢的影响[J].农业环境科学学报,2024,43(4):732-740. |
| 腐植酸负载纳米零价铁对镉污染农田水稻籽粒代谢的影响 |
| Effects of humic acid-supported nanoscale zero-valent iron on the metabolism of rice grains in cadmiumcontaminated farmland |
| 投稿时间：2023-12-11 |
| DOI：10.11654/jaes.2023-1058 |
| 中文关键词: 镉 腐植酸负载纳米零价铁 水稻 代谢组学 差异代谢物 |
| 英文关键词: cadmium humic acid supported nano zero-valent iron rice metabolomic differential metabolite |
| 基金项目:国家自然科学基金项目（42307024，22306149）；浙江省自然科学基金项目（LQ23D030007）；浙江省科技创新领军人才项目（2021R52045） |
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| 中文摘要: |
| 为探究腐植酸负载纳米零价铁（nZVI@HA）对镉（Cd）污染修复过程中水稻籽粒营养物质合成过程的影响，本研究采用吸附络合-液相还原法制备了一种nZVI@HA材料，同时开展水稻盆栽实验，利用电感耦合等离子体质谱及超高效液相色谱质谱技术，比较研究了nZVI@HA、纳米零价铁（nZVI）、腐植酸（HA）及nZVI与HA联合处理对Cd污染修复过程中水稻产量、籽粒中Cd含量及籽粒代谢物质组成的影响。结果表明：3组含Fe0处理均可提升水稻产量、降低水稻籽粒Cd的赋存水平。尤其是在nZVI@HA作用下，水稻产量显著提升至空白组的188%、籽粒Cd含量降至0.155 mg·kg-1，低于我国大米Cd限量标准（0.2 mg·kg-1，GB 2762—2022）。代谢组学分析显示，各含Fe0处理均可显著促进水稻籽粒倍他林生物合成及精氨酸和脯氨酸代谢（P<0.05且VIP>1）；同时Cd污染修复过程中，水稻籽粒还可通过丙氨酸、天冬氨酸和谷氨酸代谢，核苷酸代谢，甘氨酸、丝氨酸和苏氨酸代谢，及甘油磷脂代谢特异性响应nZVI@HA处理。研究表明，nZVI@HA可特异性作用于水稻籽粒代谢过程，提升水稻抗性及产量。 |
| 英文摘要: |
| In our study, a humic acid（HA）supported nanoscal zero valent iron（nZVI）material（nZVI@HA）was prepared by adsorption complexation-liquid phase reduction method to reveal the mechanism of the nutrients synthetic process in rice grains during the cadmium （Cd）remediation process. The effects of single nZVI@HA, nZVI, HA, and the mixture of nZVI and HA on rice yield, Cd content in grains, and metabolite compositions of grains in the soil Cd remediation process, were studied. Here, we conducted a series of rice pot experiments equipped with ultra-high-performance liquid chromatography-mass spectrometry technology and inductively coupled plasma mass spectrometry. The results demonstrated that the three Fe0 treatments could increase rice yield and reduce the Cd content in rice grains. Especially under the application of nZVI@HA, the rice yield was significantly increased to 188% of control, and the Cd content in grains was reduced to 0.155 mg·kg-1 lower than the national Cd limit standard for rice（0.2 mg·kg-1, GB 2762—2022）. Metabolomics analysis showed that the three Fe0-containing treatments could significantly promote betalain biosynthesis and the arginine and proline metabolism in grains（P<0.05 and VIP>1）. Notably, the grains could also respond highly and specifically to nZVI@HA through alanine, aspartate, and glutamate metabolism, nucleotide metabolism, glycine, serine and threonine metabolism, and glycerophospholipid metabolism. Above all, nZVI@HA has specific effects on grain metabolisms, leading to improvements in rice resistance and yield. |
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