文章摘要
吴晓梅,叶美锋,吴飞龙,徐庆贤,林代炎.铜在狐尾藻中的积累及亚细胞分布和化学形态[J].农业环境科学学报,2024,43(5):1114-1122.
铜在狐尾藻中的积累及亚细胞分布和化学形态
Accumulation,subcellular distribution,and chemical forms of copper in Myriophyllum spicatum L.
投稿时间:2023-10-30  
DOI:10.11654/jaes.2023-0899
中文关键词: 狐尾藻    亚细胞分布  化学形态  耐性机制
英文关键词: Myriophyllum spicatum L.  copper  subcellular distribution  chemical form  tolerance mechanism
基金项目:福建省自然科学基金项目(2021J01497);福建省省属公益类科研院所专项(2021R1032003,2021R1032004,2021R1032005)
作者单位E-mail
吴晓梅 福建省农业科学院农产品加工研究所, 福州 350003  
叶美锋 福建省农业科学院农产品加工研究所, 福州 350003  
吴飞龙 福建省农业科学院农产品加工研究所, 福州 350003  
徐庆贤 福建省农业科学院农产品加工研究所, 福州 350003  
林代炎 福建省农业科学院农产品加工研究所, 福州 350003 lindaiyan@126.com 
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中文摘要:
      为探索狐尾藻对重金属铜的积累和耐性机制,本研究通过水培试验,研究不同浓度铜处理(0、20、50 mg·L-1)对狐尾藻(Myriophyllum spicatum L.)生长生理特性以及叶片表皮细胞形态的影响,分析各器官中铜吸收转运及铜在各组织器官亚细胞中的分布和化学形态。结果表明:各浓度铜处理下狐尾藻均能存活,但铜浓度高于50 mg·L-1时,狐尾藻根、茎、叶生物量相比对照(铜 0 mg·L-1)处理降低53.48%、36.99%和32.22%。铜处理后,狐尾藻根、茎和叶铜含量分别为11.81~186.34、1.32~7.89、2.11~11.99 mg·kg-1,根系中铜含量均高于叶片和茎部。铜在狐尾藻中的亚细胞分布主要位于根、茎、叶的细胞壁部分(36.49%~49.61%、45.44%~49.92%、41.45%~55.92%),其次是可溶性组分(21.65%~25.99%、23.03%~27.65%、18.01%~34.63%)。狐尾藻中铜的赋存化学形态以盐酸提取态、醋酸提取态和乙醇提取态为主,所占比例为76.34%~86.67%,均是活性较低的形态。因此,狐尾藻是铜富集较好的植物,其根部的耐性大于茎、叶。铜以吸附态或蛋白质、果胶酸盐等低活性形态赋存于细胞壁或可溶性组分(液泡)中是狐尾藻积累和耐受铜的重要机制。
英文摘要:
      To explore the accumulation and tolerance mechanism in Myriophyllum spicatum L., a hydroponic experiment was conducted to investigate its growth, physiological response, and copper uptake translocation. The subcellular distribution and chemical form of copper in roots, stems, and leave were further analyzed. M. spicatum could survive under different copper treatments. However, when the copper concentration exceeded 50 mg·L-1, the biomass of roots, stems, and leave of M. spicatum decreased by 53.48%, 36.99%, and 32.22%, respectively, compared to that under the control treatment(copper 0 mg·L-1). Following copper treatment, the copper contents in the roots, stems, and leave of M. spicatum were 11.81–186.34 mg·kg-1, 1.32–7.89 mg·kg-1, and 2.11–11.99 mg·kg-1, respectively, with higher contents in the roots than in the shoots. The subcellular distribution of copper in M. spicatum was mainly located in the cell wall parts of the roots, stems, and leave(36.49%–49.61%, 45.44%–49.92%, and 41.45%–55.92%, respectively), followed by the soluble component(21.65%–25.99%, 23.03%–27.65%, and 18.01%-34.63%, respectively). The copper existed in HCl-, HAc-, and ethanol-extracted forms in M. spicatum, accounting for 76.34% to 86.67%, all with low activity. These results show that M. spicatum has good copper accumulation, and its root tolerance is greater than that of stems and leaves. The primary resistance mechanism underlying copper accumulation and tolerance in M. spicatum might involve the presence of copper in the cell wall or soluble components(vacuoles) in an adsorbed state or a low-active form, such as protein or pectate.
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