文章摘要
李煜,叶青青,瞿云山,刘子墨,任玉莹,于莉民,李欣.纳米铁氧化物强化牛粪厌氧发酵产甲烷[J].农业环境科学学报,2024,43(5):1163-1170.
纳米铁氧化物强化牛粪厌氧发酵产甲烷
Enhanced methane production from anaerobic digestion of cattle manure by adding iron oxide nanoparticles
投稿时间:2023-10-12  
DOI:10.11654/jaes.2023-0828
中文关键词: 厌氧发酵  牛粪  纳米铁氧化物  挥发性脂肪酸  宏基因组学
英文关键词: anaerobic digestion  cow manure  iron oxide nanoparticle  volatile fatty acid  metagenomic analysis
基金项目:中国博士后面上项目(2022M713393);博士后国际交流引进项目(YJ20210197)
作者单位
李煜 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
叶青青 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
瞿云山 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
刘子墨 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
任玉莹 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
于莉民 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
李欣 中国农业大学工学院农业部可再生能源清洁化利用技术重点实验室, 北京 100083 
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中文摘要:
      为探究金属添加剂对牛粪厌氧发酵产甲烷过程的影响,提升牛粪厌氧发酵效率,本研究以纳米铁氧化物(Iron oxide nanoparticle,INP)为典型金属添加剂,比较了添加INP的反应器和从未添加INP的对照反应器中日产甲烷量、总挥发性酸、纤维素和半纤维素等的变化,并进一步探究INP添加对微生物群落和关键酶活性的影响。结果显示,INP添加促进纤维素水解并有利于挥发性脂肪酸的降解,从而促进产甲烷过程。连续反应器每日添加625 mg∙L-1 INP时日甲烷产量达到173.4 mL∙g-1∙d-1,比对照组提高了38.3%;即使反应器停止添加INP,与对照组相比日产甲烷量依然显著提高(P<0.05)。16S rRNA分析表明,INP添加富集了乙酸氧化菌Mesotoga和嗜氢产甲烷菌Methanoculleus这一对协同互营菌。宏基因组分析表明,INP添加显著促进嗜乙酸产甲烷途径和嗜氢产甲烷途径相关酶的活性,尤其提高了甲烷合成关键酶-甲基辅酶M还原酶(EC 2.8.4.1)的活性(P<0.05),这是产甲烷效能提升的主要原因。综上,INP添加剂的施用有利于牛粪厌氧发酵产甲烷,从而提升畜禽粪污能源化利用效率。
英文摘要:
      To investigate the influence of metal additives on the anaerobic digestion of cattle manure and enhance fermentation efficiency, this study chose iron oxide nanoparticles(INP) as a representative metal additive. We compared the changes in daily methane production, total volatile acids, cellulose, and hemicellulose between reactors with INP added and control reactors that never received INP. Additionally, the impact of INP addition on microbial communities and key enzyme activities was further explored through microbial analysis. The results demonstrated that INP addition promoted cellulose hydrolysis and facilitated the degradation of volatile fatty acids, thereby enhancing methane production. When 625 mg∙L-1 of INP was added daily to the continuous reactor, the daily methane production reached 173.4 mL∙g-1∙d-1, 38.3% higher than the control group. Even when the addition of INP ceased, methane production remained significantly higher compared with the control group(P<0.05). 16S rRNA analysis revealed the enrichment of a pair of syntrophic microbial guilds Mesotoga, which oxidizes acetic acid, and Methanoculleus, a hydrogenotrophic methanogen. Metagenomic analysis revealed that the addition of INP significantly enhanced the activities of enzymes associated with both acetoclastic and hydrogenotrophic methanogenesis, particularly increasing the activity of the key enzyme in methane synthesis, methyl-coenzyme M reductase(EC 2.8.4.1)(P<0.05). This was identified as the primary reason for the increased methane production efficiency. Thus, the application of metal additives represented by INP is beneficial for methane production in anaerobic fermentation of cattle manure, thereby improving the efficiency of livestock manure energy utilization.
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