| .全氟辛酸和雌二醇对黄孢原毛平革菌过氧化物酶活性的影响[J].农业环境科学学报,2013,32(6):. |
| 全氟辛酸和雌二醇对黄孢原毛平革菌过氧化物酶活性的影响 |
| Effects of Perfluorooctanoic Acid and Estradiol on the Peroxidase Enzyme Activity of Phanerochaete chrysosporium |
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| DOI：10.11654/jaes.2013.06.008 |
| 中文关键词: 黄孢原毛平革菌 纯培养 过氧化物酶活性 全氟辛酸 雌二醇 |
| 英文关键词: Phanerochaete chrysosporium pure culture activity of peroxides enzyme PFOA E2 |
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| 摘要点击次数: 1778 |
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| 中文摘要: |
| 选取典型的持久性有机污染物全氟辛酸（ＰＦＯＡ）和雌二醇（Ｅ２），采用Ｋｉｒｋ液体培养基对黄孢原毛平革菌（Ｐｈａｎｅｒｏｃｈａｅｔｅ ｃｈｒｙｓｏｓｐｏｒｉｕｍ，简称ＰＣ菌）纯培养的方式，以分光光度法测定了该体系中菌液总酶活性的变化规律，研究了ＰＦＯＡ和Ｅ２对其胞外过氧化物酶活性的影响。结果表明：（１）以“２，２－联氮基双（３－乙基苯并噻唑啉－６－磺酸）二铵盐”（ＡＢＴＳ）为底物，在ｐＨ为３的柠檬酸盐缓冲液中，依次加入ＡＢＴＳ、Ｈ２Ｏ２、ＭｎＳＯ４和菌液，在４７０ ｎｍ处测定６０ ｓ 内反应的酶活性是其最大值，包含木质素过氧化物酶（ＬｉＰ）、锰过氧化物酶（ＭｎＰ）和漆酶（Ｌａｃ）等胞外酶的总活性。（２）该培养体系中，ＰＣ菌分泌的胞外酶以ＬｉＰ和ＭｎＰ两种过氧化物酶为主，Ｌａｃ活性极低；总酶活性随着培养时间的增加而增加，在第２０~３０ d之间出现一次小高峰，５０~６０ d之间出现一次大高峰，随后活性下降。（３）０．０１ ｍｍｏｌ·Ｌ-1的全氟辛酸（ＰＦＯＡ）对这两种过氧化物酶（ＬｉＰ和ＭｎＰ）活性及菌液总酶活性的影响是一致的，表现为“先抑制，后促进”的作用；０．１ μｍｏｌ·Ｌ-1的雌二醇（Ｅ２）对其影响也是一致的，均表现为抑制作用。 |
| 英文摘要: |
| Phanerochaete chrysosporium（PC） was pure cultured with Kirk liquid medium, two typical persistent organic pollutants perfluorooctanoic acid（PFOA） and estradiol（E2） were chosen, the effects of PFOA and E2 on PC extracellular peroxidase activity and total enzyme activities were studied. The results showed that 2, 2′-Azino-bis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt（ABTS）was used as the substrate, and ABTS, H2O2, MnSO4, fungi solution were added in turn to citrate buffer with pH 3 to form a solution. The maximum bacterium activity was measured at 470 nm within 60 s reaction, which contained total activity of extracellular enzymes including lignin peroxidase（LiP）, manganese peroxidase（MnP）, laccase（Lac） and so on. Lip and MnP were two main extracellular enzymes secreted by PC in the culture system, while Lac enzyme activity was very low. Total enzyme activity increased with incubation time of PC. A small peak was observed between 20~30 days, while a big peak appeared between 50~60 days, and which decreased thereafter. Effects on activities of the two peroxidases（LiP and MnP） and total enzymes activities of broth were the same with addition of 0.01 mmol·L-1 PFOA, which was inhibited at beginning and promoted later. Effects with addition of 0.1 mmol·L-1 E2 on activities of the two peroxidases（LiP and MnP）and total enzyme activities of broth were the same, which executed consistent inhibition effects. |
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