| .石油污染土壤生物修复对土壤酶活性的影响[J].农业环境科学学报,2013,32(6):. |
| 石油污染土壤生物修复对土壤酶活性的影响 |
| Effect of Soil Enzyme Activities During Bioremediation of Crude Oil-Contaminated Soil |
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| DOI：10.11654/jaes.2013.06.014 |
| 中文关键词: 土壤 石油 生物修复 土壤酶活性 总石油烃 |
| 英文关键词: soil crude oil bioremediation soil enzyme activity total petroleum hydrocarbon |
| 基金项目: |
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| 摘要点击次数: 2193 |
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| 中文摘要: |
| 土壤酶活性是土壤微生物生物化学过程的综合反映。为了探究石油污染土壤生物修复过程中土壤酶活性变化规律研究，采用筛选和分离的3株对石油烃有良好降解效果的降解菌构建了混合菌体系，开展了石油污染土壤模拟生物修复实验，考察了不同修复时期土壤石油烃残留量、石油烃表观降解率以及四种土壤酶（脲酶、过氧化氢酶、脱氢酶、脂肪酶）活性的变化。结果表明，石油污染土壤经40 d生物修复后，石油烃表观降解率达到64.4%。在石油污染土壤的生物修复过程中，脲酶活性在0~24 d上升较快，24 d后趋于稳定；过氧化氢酶和脱氢酶活性的变化规律相似，均在前期上升随后略有下降；脂肪酶活性有一个快速上升阶段（0~16 d），而后又出现明显的下降。进一步统计分析表明，脲酶与石油烃残留量呈显著负相关性（r=-0.916，P＜0.05）；过氧化氢酶和脱氢酶活性与石油烃残留量呈极显著负相关性，相关系数分别为-0.974（P＜0.01）和-0.969（P＜0.01）；而脂肪酶活性与石油烃残留量的相关性不显著（P>0.05）。 |
| 英文摘要: |
| Soil enzyme activity comprehensively reflects the soil microorganism biochemical process. In order to explore the changes of soil enzyme activities during bioremediation of crude oil-contaminated soil,constructed crude oil degrading bacterial consortium were built withthree strains isolated from oil-contaminated soil, bioremediation experiments of crude oil-contaminated soil were conducted, and the change of total petroleum hydrocarbon（TPH）, apparent degradation efficiencies and soil enzyme activities（urease, catalase, dehydrogenase and lipase） were examined during the crude oil-contaminated soil bioremediation. The results showed that the apparent degradation efficiency of crude oil reached 64.4% after 40 d of bioremediation. Urease activity rapidly increased during 0~24 d, then tended to be stable.The catalase activity and dehydrogenase activity increased in early stage then slightly declined. However, lipase activity had a rapid rise phase（0~16 d）, then obviously slowed down. The results of statistical analysis showed that soil urease activity and TPH had significant negative relationship（-0.916, P＜0.05）. Catalase and dehydrogenase activities were both significantly correlated with soil TPH concentration, and their correlation coefficients were -0.974（P＜0.01） and -0.969（P＜0.01）, respectively. However, soil lipase activity did not have significant correlationship with TPH（P>0.05）. Therefore, soil enzyme activities are potential valuable indicators of oil biodegradation. |
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