| .脱氮副球菌硝酸盐/亚硝酸盐还原酶的活性变化及对养殖水体中无机氮素的转化[J].农业环境科学学报,2013,32(6):. |
| 脱氮副球菌硝酸盐/亚硝酸盐还原酶的活性变化及对养殖水体中无机氮素的转化 |
| The Changes of Nitrate and Nitrite Reductase Activity of Paracoccus denitrificans and Its Inorganic Nitrogen Removal for Aquaculture Water |
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| DOI：10.11654/jaes.2013.06.023 |
| 中文关键词: 脱氮副球菌 反硝化 硝酸盐还原酶 亚硝酸盐还原酶 养殖水体 无机氮 |
| 英文关键词: Paracoccus denitrificans denitrification nitrate reductase nitrite reductase aquaculture water inorganic nitrogen |
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| 中文摘要: |
| 以一株脱氮副球菌（Paracoccus denitrificans）为试验菌株，研究了其在不同溶氧条件下菌体内硝酸盐还原酶和亚硝酸盐还原酶活性的变化及对水体中无机氮的转化。结果表明，脱氮副球菌是一株好氧细菌，当水体中存在碳源及3种不同形式的无机氮时，水体DO的水平决定了菌体的生长速率及对无机氮的转化利用效率。DO充足，菌体生长速率快，细胞量高，反之则生长缓慢。脱氮副球菌可有效转化不同形式的无机氮，其以同化的方式转化NH+4-N，而以反硝化方式转化去除NO-2-N和NO-3-N。反硝化作用的程度取决于细菌体内异化型硝酸盐还原酶和亚硝酸盐还原酶的活力。而异化型硝酸盐还原酶和亚硝酸盐还原酶的活力受到水体中NO-3-N和NO-2-N的含量以及DO水平的显著影响。从转化速率和效能上来看，水体DO至少应保持在3.6 mg·L-1以上。将活菌数≥109个·mL-1的脱氮副球菌按0.75、1.50、2.25 mg·L-1的用量加入淡水池塘养殖水体，在38 d试验期内可使养殖水体中的NH+4-N下降39.71%~49.33%，NO-2-N下降50.00%~64.39%，NO-3-N下降44.44%~60.00%，高锰酸盐指数下降7.81%~21.49%，对养殖水体中的无机氮素及有机物污染具有较好的控制效果。研究显示，脱氮副球菌的好氧反硝化作用可以为养殖水体有氧条件下的脱氮提供一条新的思路。 |
| 英文摘要: |
| This paper focused on the activities of nitrate reductase and nitrite reductase of Paracoccus denitrificans and its inorganic nitrogen control for aquaculture water under different dissolved oxygen conditions. The results showed that Paracoccus denitrificans was an aerobic bacteria, and when carbon sources and three different forms of inorganic nitrogen coexistenced in water, the concentration of DO determined the rate of cell growth and the transformation efficiency of inorganic nitrogen. The higher the DO concentration, the faster the growth, the bigger the cells biomass. Paracoccus denitrificans had good conversion efficiency for different forms of inorganic nitrogen, it always transformed NH+4-N via assimilation, while almost transformed all NO-3-N and NO-2-N via denitrification. The degree of denitrification depended on the vitality of in vivo dissimilatory nitrate reductase and nitrite reductase of Paracoccus denitrificans. And the vitality of these two enzymes was significantly affected by the concentration of NO-3-N, NO-2-N and DO. Judging from the aspects of conversion rate and efficiency, the concentration of water DO should be kept at least 3.6 mg·L-1. Paracoccus denitrificans（viable counts≥109·mL-1） was used to control the nitrogen pollution of aquaculture water , when the concentration of Paracoccus denitrificans in aquaculture water reached to 0.75 mg·L-1, 1.50 mg·L-1 and 2.25 mg·L-1, the NH+4-N of aquaculture water decreased by 39.71%~49.33%, NO-2-N decreased by 50.00%~64.39%, NO-3-N decreased by 44.44%~60.00%, CODMn decreased by 7.81%~21.49% within 38 days. The results indicated that Paracoccus denitrificans could effectively control nitrogen and organic pollution of aquaculture water. The details of the aerobic denitrification of Paracoccus denitrificans provided a new idea for nitrogen removal of aquaculture water. |
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