文章摘要
梁忠秀,李 健,刘 萍,李吉涛,赵法箴.塔玛亚历山大藻对中国明对虾免疫相关基因表达和抗病力的影响[J].农业环境科学学报,2013,32(11):2271-2277.
塔玛亚历山大藻对中国明对虾免疫相关基因表达和抗病力的影响
Influences of Toxic Dinoflagellate Alexandrium tamarense on Relative Expression of Immune Related Genes and Disease Resistance in Fenneropenaeus chinensis
  
DOI:10.11654/jaes.2013.11.024
中文关键词: 中国明对虾  塔玛亚历山大藻  TLR  Relish  抗病力
英文关键词: Fenneropenaeus chinensis  Alexandrium tamarense  TLR  Relish  disease resistance
基金项目:
作者单位
梁忠秀 上海海洋大学水产与生命学院 上海 200306中国水产科学研究院 黄海水产研究所 山东 青岛 266071 
李 健 中国水产科学研究院 黄海水产研究所 山东 青岛 266071 
刘 萍 中国水产科学研究院 黄海水产研究所 山东 青岛 266071 
李吉涛 中国水产科学研究院 黄海水产研究所 山东 青岛 266071 
赵法箴 中国水产科学研究院 黄海水产研究所 山东 青岛 266071 
摘要点击次数: 2423
全文下载次数: 2062
中文摘要:
      以一株能产生麻痹性贝毒(Paralytic Shellfish Poison,PSP)的赤潮甲藻塔玛亚历山大藻(Alexandrium tamarense,ATHK株)为研究对象,研究了其对中国明对虾(Fenneropenaeus chinensis)免疫相关基因(TLR和Relish基因)表达和抗病力的影响。结果表明:200 cells·mL-1塔玛亚历山大藻胁迫72~144 h,中国明对虾的肝胰腺、鳃和血淋巴的TLR和Relish 基因表达或被显著抑制(P<0.05),或恢复至海水对照组的水平(P>0.05);而1000 cells·mL-1藻中对虾鳃组织和血淋巴的上述基因表达在48~144 h被显著抑制(P<0.05),肝胰腺的表达在72~144 h被显著抑制(P<0.05)。塔玛亚历山大藻胁迫下投喂感染WSSV的病虾后,加藻的感染组和未加藻的感染组的中国明对虾虽然都在第3 d时检测到阳性结果,7 d内累积死亡率为100%,但同一时间点加藻组的累积死亡率高于未加藻组。塔玛亚历山大藻胁迫6 d后的中国明对虾,经鳗弧菌(Vibrio anguillarum)人工急性感染后,两个加藻组在同一取样时间点的累积死亡率高于未加藻的海水对照组,且随着藻浓度升高而有升高的趋势。研究表明塔玛亚历山大藻可影响中国明对虾TLR和Relish基因表达,从而影响机体的抗病力。
英文摘要:
      Dinoflagellate Alexandrium tamarense(ATHK), a producer of paralytic shellfish poison, is often found in Fenneropenaeus chinensis ponds. Here we studied the influence of A. tamarense on relative expressions of immune related genes(TLR gene and Relish gene)and disease resistance in the Chinese shrimp F. chinensis. Individuals of F. chinensis were exposed to 200 cells·mL-1 and 1000 cells·mL-1 A. tamarense, and their TLR gene and Relish gene expressions were then determined at 3, 6, 12, 24, 48, 72, 96 h and 144 h. Under exposure to 200 cells·mL-1 A. tamarense, the relative expressions of TLR gene and Relish gene were not consistently inhibited in the hepatopancreas, gill and hemolymph of the shrimp between 72 h and 144 h. However, such expressions were significantly inhibited under exposure to 1000 cells·mL-1 A. tamarense in the gill and hemolymph within 48~144 h and in the hepatopancreas between 72 h and 144 h. After the individuals of F. chinensis pre-exposed to A. tamarense(200 cells·mL-1 and 1000 cells·mL-1) were artificially infected with WSSV, a positive infection by the WSSV was detected after 3 days. The cumulative mortality of F. chinensis was 100% after 7 days for exposure to both A. tamarense and seawater control, but was higher in the A. tamarense group than in the seawater control group at the same sampling time. In addition, when shrimps were exposed to A. tamarense for 6 days, followed by infection with Vibrio anguillarum, their cumulative mortality at the same sampling time was still higher in the A. tamarense group than that in the seawater control. Therefore,it is suggested that A. tamarense affect the relative expressions of TLR and Relish genes and disease resistance in F. chinensis.
HTML    查看全文   查看/发表评论  下载PDF阅读器