苄嘧磺隆对水稻田土壤微生物群落功能多样性的影响

张雯雯; 徐 军; 董丰收; 刘新刚; 向文胜; 郑永权

文章摘要

张雯雯,徐军,董丰收,刘新刚,向文胜,郑永权.苄嘧磺隆对水稻田土壤微生物群落功能多样性的影响[J].农业环境科学学报,2014,33(9):1749-1754.

苄嘧磺隆对水稻田土壤微生物群落功能多样性的影响

Responses of Microbial Community Functional Diversity to Bensulfuron-methyl in Paddy Soil

DOI：10.11654/jaes.2014.09.012

中文关键词: 苄嘧磺隆微生物群落 BIOLOG 功能多样性水稻田土壤

英文关键词: bensulfuron-methyl microbial community BIOLOG functional diversity paddy soil

基金项目:

作者	单位
张雯雯	东北农业大学农学院，哈尔滨 150030；中国农业科学院植物保护研究所，农业部作物有害生物综合治理重点实验室，北京 100193
徐军	中国农业科学院植物保护研究所，农业部作物有害生物综合治理重点实验室，北京 100193
董丰收	中国农业科学院植物保护研究所，农业部作物有害生物综合治理重点实验室，北京 100193
刘新刚	中国农业科学院植物保护研究所，农业部作物有害生物综合治理重点实验室，北京 100193
向文胜	东北农业大学农学院，哈尔滨 150030
郑永权	中国农业科学院植物保护研究所，农业部作物有害生物综合治理重点实验室，北京 100193

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中文摘要:

采用BIOLOG碳源底物利用法研究了不同浓度苄嘧磺隆（空白对照0 mg·kg-1、田间最大推荐剂量0.4 mg·kg-1和10倍田间最大推荐剂量4 mg·kg-1）对水稻田土壤微生物群落功能多样性的影响。对BIOLOG微平板每孔颜色平均变化率（AWCD值）的分析结果表明，低浓度苄嘧磺隆会提高微生物的碳源利用能力，高浓度苄嘧磺隆表现抑制作用，且随着时间延长，苄嘧磺隆对微生物的促进和抑制作用逐渐消失。施药后96 h水稻田土壤微生物群落功能多样性指数（Shannon指数、Simpson指数和McIntosh指数）的数据分析结果表明，苄嘧磺隆会导致水稻田土壤微生物群落的碳源利用能力集中、可利用碳源种类数减少以及碳源利用程度下降。对水稻田土壤微生物群落主成分分析结果表明，苄嘧磺隆改变了水稻田土壤微生物的群落结构，但随着时间延长，苄嘧磺隆对水稻田土壤微生物群落结构的影响逐渐消失。

英文摘要:

A laboratory incubation study was conducted to ascertain the effects of bensulfuron-methyl on soil microbial community functional diversity in a paddy soil. Bensulfuron-methyl was applied to soil at three rates：0（control）, 0.4 mg·kg-1 dry weight soil（the maximum recommended field rate） and 4 mg·kg-1 dry weight soil（ten-fold of the maximum recommended field rate）. Soil microbial community functional diversity indexes with potential as bioindicators of soil health were measured using BIOLOG Ecoplates at different times after herbicide application. The average well color development（AWCD） indicated that the carbon source substrates utilization ability of microbial communities were enhanced at low application rates but inhibited at high application rates. However, bensulfuron-methyl did not show significant difference from the control soils as exposure time increased. At 96 h, Shannon index, Simpson index and McIntosh index showed that bensulfuron-methyl significantly decreased utilization ability and efficiency of carbon substrates, and number of utilizable carbon substrate species by the microbial communities. Principal component analysis（PCA） indicated the soil microbial community structure was shifted by bensulfuron-methyl application, but recovered at the end of experiment. These results suggest that bensulfuron-methyl application shifts the soil microbial community structure and function, but they tend to recover as time extends.

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