| 曹庭悦,刘鸣达,沃惜慧,李军.硅、磷配施对水稻镉吸收转运的影响及其机制[J].农业环境科学学报,2020,39(1):37-44. |
| 硅、磷配施对水稻镉吸收转运的影响及其机制 |
| Effects of combined application of silicon and phosphorus on cadmium uptake and transport in rice and its mechanisms |
| 投稿时间：2019-07-17 |
| DOI：10.11654/jaes.2019-0796 |
| 中文关键词: 水稻 硅 磷 镉 基因相对表达量 |
| 英文关键词: rice silicon phosphorus cadmium relative expression of genes |
| 基金项目:国家自然科学基金项目（41371306） |
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| 摘要点击次数: 2061 |
| 全文下载次数: 1706 |
| 中文摘要: |
| 为了探究硅、磷配施对水稻镉吸收、转运的影响及其调控机制，采用水培试验，以沈稻529为研究对象，在镉污染胁迫下，各施入4种浓度梯度的硅、磷处理，培养30 d后，取水稻样品对硅、磷施入后水稻生物量、地上部及地下部镉含量、根茎转移系数以及水稻镉转运相关基因表达进行比较分析。随着硅、磷的配合施入水稻生物量增加，当284 mg·kg-1浓度的硅与180 mg·kg-1浓度的磷配合施入时水稻地上部及地下部镉含量、根茎转移系数达到最低。随着硅、磷的施入，水稻OsLCT1和LCD基因表现出基因相对表达量下调的趋势，OsNramp5基因表现出基因相对表达量上调的趋势，且配合施入调控幅度大于单一元素施入调控幅度。硅、磷的施入可以促进镉胁迫下水稻的生长，并通过调控水稻中镉相关基因的表达来实现对水稻中镉吸收、转移的影响。 |
| 英文摘要: |
| In order to explore the effects of silicon and phosphorus on the absorption and transport of cadmium in rice and its regulation mechanism. Shendao 529 was used as the research object in hydroponic experiments. In addition, four concentration gradients of silicon and phosphorus were applied under cadmium pollution stress, After 30 days of cultivation, rice samples were used to compare the biomass of rice, the cadmium content in shoots and below ground, the rhizosphere transfer coefficient, and the expression of cadmium transport related genes in rice.It was found that with the increase of silicon and phosphorus application in rice, lower cadmium content and rhizome transfer coefficients of the above-ground and underground parts of rice were achieved, specifically when combined with the silicon concentrations of 284 mg·kg-1 and phosphorus of 180 mg·kg-1.With the application of silicon and phosphorus, the rice OsLCT1 and LCD genes showed a downward trend in their relative expression. The OsNramp5 gene showed a trend of up-regulation in its relative expression, regulation range of the application was greater than that of the single element. It was therefore determined that the application of silicon and phosphorus can promote the growth of rice under cadmium stress and can affect the cadmium uptake and transfer in rice by regulating the expression of cadmium-related genes. |
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