| 万文,杨天杰,樊晓腾,郑海平,王世梅,韦中,徐阳春,沈其荣.促进草酸青霉降解秸秆的表面活性剂效果研究[J].农业环境科学学报,2022,41(7):1582-1589. |
| 促进草酸青霉降解秸秆的表面活性剂效果研究 |
| Effects of surfactants on promotion of straw degradation by Penicillium oxalicum |
| 投稿时间：2021-12-15 |
| DOI：10.11654/jaes.2021-1453 |
| 中文关键词: 表面活性剂 秸秆 降解 草酸青霉 |
| 英文关键词: surfactant straw degradation Penicillium oxalicum |
| 基金项目:中国博士后科学基金项目(2019M650116);江苏省博士后科研资助计划(2018K200C);江苏省研究生科研创新计划项目(KYCX17_0593) |
|
| 摘要点击次数: 987 |
| 全文下载次数: 799 |
| 中文摘要: |
| 为了提高秸秆降解效率,本文探究了不同种类表面活性剂对秸秆降解菌降解秸秆的作用,并筛选出效果最佳的表面活性剂。首先筛选获得一株降解玉米秸秆能力较强的菌株——草酸青霉Z7-6;其次在液体发酵条件下,考察4种表面活性剂(两个浓度)对草酸青霉Z7-6降解玉米秸秆效果及产纤维素类酶的影响;最终通过固体发酵验证表面活性剂的效果。结果表明:筛选出的草酸青霉Z7-6对玉米秸秆具有较好的降解效果,相对降解率达40.58%。液体发酵条件下,0.1%表面活性剂处理下秸秆相对降解率较高,0.1% Triton X-100(TR)和0.1% Tween 80(TW)均可提升草酸青霉Z7-6降解玉米秸秆的效果,两者间无显著差异。0.1% TR处理中滤纸酶活性和羧甲基纤维素酶活性均显著高于其他处理。固体发酵条件下,0.1% TR的提升效果最佳。综合秸秆降解的效果和产酶情况,发现0.1% TR促进草酸青霉Z7-6降解秸秆的效果最好,也可提升该菌株纤维素类酶的活性。 |
| 英文摘要: |
| In order to improve degradation efficiency, effects of different types of surfactants on straw degradation by straw-degrading bacteria were explored and the best surfactant were screened out. We first isolated a strain, Penicillium oxalicum Z7-6, with strong ability of degrade corn straw. Under liquid fermentation, effects of four surfactants (with two concentrations) on the degradation of corn straw by P. oxalicum Z7-6 and the production of cellulosic enzymes were investigated. Finally, the effects of the surfactants were verified by solid fermentation. The isolated P. oxalicum Z7-6 could degrade corn straw with high efficiency at a relative degradation rate of 40.58%. Under liquid fermentation condition, the relative degradation rate of straw was higher after treated with surfactants at a concentration of 0.1%. Both 0.1% Triton X-100 (TR) and 0.1% Tween 80 (TW) could significantly promote degradation of corn straw by P. oxalicum Z7-6, and there was no significant differences between the effects of these two treatments. Activities of filter paper enzyme and carboxymethyl cellulase were significantly higher under 0.1% TR compared with other treatments. Under solid fermentation, straw degradation was significantly higher under 0.1% TR compared with other treatments. Thus, the results for straw degradation and enzyme activities show that 0.1% TR has the strongest promotion effect on the degradation of straw by P. oxalicum Z7-6 and cellulosic enzyme activities. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
