| 庄捷,赵斌成,薛锦辉,张文艺.猪粪厌氧消化进程中雌激素的去除效能及机制[J].农业环境科学学报,2023,42(2):461-471. |
| 猪粪厌氧消化进程中雌激素的去除效能及机制 |
| Estrogen removal and its mechanism during anaerobic digestion of pig manure |
| 投稿时间：2022-03-21 |
| DOI：10.11654/jaes.2022-0262 |
| 中文关键词: 厌氧消化 雌激素 溶解性有机物 微生物群落结构 |
| 英文关键词: anaerobic digestion estrogen dissolved organic matter microbial community structure |
| 基金项目:江苏省科技支撑计划项目（BE2020761）；江苏省研究生科研与实践创新计划项目（SJCX20-0942） |
|
| 摘要点击次数: 825 |
| 全文下载次数: 769 |
| 中文摘要: |
| 为探究猪粪厌氧消化进程中雌激素的去除效能及其作用机制，对厌氧消化液中的4种雌激素（雌酮E1、雌二醇E2、雌三醇E3、炔雌醇EE2）进行阶段性检测，并采用傅里叶变换红外光谱、三维荧光光谱结合荧光区域积分和16S rRNA基因扩增子高通量测序的手段对猪粪中红外官能团振动特征峰、溶解性有机物（DOM）和微生物群落结构进行分析。结果表明：在温度为35℃、周期为30 d、固体浓度为6%的条件下，厌氧消化对4种雌激素的降解速率依次为E2>E3>E1>EE2，去除效率分别为28.62%、25.83%、19.14%、11.81%，降解规律均符合有机物一级动力学降解模型。在投加雌激素之后，猪粪中微生物群落结构发生了一定改变，但仍能进行正常的厌氧消化，并促使与雌激素相结合的腐植酸类溶解性有机物含量增加，同时其中还存在酰胺、羟基、羧基等雌激素吸附位点以及雌激素高效降解菌。研究推测沼液中雌激素的去除机制包含猪粪颗粒的吸附、溶解性有机物的吸附和雌激素降解菌属的高效代谢3个方面。 |
| 英文摘要: |
| To investigate the estrogen removal efficiency and its mechanism during the anaerobic digestion of pig manure, four estrogens (estrone E1, estradiol E2, estriol E3, and ethinylestradiol EE2) in anaerobic digestion liquid were tested periodically. Fourier transform infrared spectroscopy, three-dimensional fluorescence spectroscopy combined with fluorescence region integration, and high-throughput sequencing of the 16S rRNA gene were used to analyze the characteristic vibration peaks of infrared functional groups, dissolved organic matter(DOM), and microbial community structure in the manure. The results showed that under a temperature of 35℃ for 30 days and 6% solid concentration condition, the degradation rates of the four estrogens by anaerobic digestion were E2>E3>E1>EE2, and the removal efficiency rates were 28.62%, 25.83%, 19.14% and 11.81%, respectively. The degradation laws were consistent with the first-order kinetic degradation model for organic matter. After the addition of estrogen, changes occurred in the microbial community of the manure; however, normal anaerobic digestion was maintained. Anaerobic digestion could also be combined with increasing estrogen humic acid soluble organic matter content and amide, hydroxyl, and carboxyl adsorption sites, and highly efficient estrogen degradation bacteria. This study speculated that the removal mechanism of estrogen from biogas slurry included three aspects:adsorption of pig manure particles, adsorption of dissolved organic matter, and efficient metabolism of estrogen-degrading bacteria. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
