文章摘要
赖丽华,田斐,熊倩,王学锋,叶国玲,陈海刚.六溴环十二烷(HBCD)和Cu2+单独与联合暴露对海水小球藻的毒性作用[J].农业环境科学学报,2023,42(7):1616-1623.
六溴环十二烷(HBCD)和Cu2+单独与联合暴露对海水小球藻的毒性作用
Single and joint toxicity of hexabromocyclododecane(HBCD)and Cu2+ on marine Chlorella salina
投稿时间:2022-12-15  
DOI:10.11654/jaes.2022-1272
中文关键词: 六溴环十二烷    海水小球藻  急性毒性  联合毒性
英文关键词: hexabromocyclododecane  copper  Chlorella salina  acute toxicity  joint toxicity
基金项目:中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项(2021SD17)
作者单位E-mail
赖丽华 中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 农业农村部南海渔业资源环境科学观测实验站, 广东珠江口生态系统野外科学观测研究站, 广州 510300
广东海洋大学水产学院, 广东 湛江 524088 
 
田斐 中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 农业农村部南海渔业资源环境科学观测实验站, 广东珠江口生态系统野外科学观测研究站, 广州 510300  
熊倩 中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 农业农村部南海渔业资源环境科学观测实验站, 广东珠江口生态系统野外科学观测研究站, 广州 510300  
王学锋 广东海洋大学水产学院, 广东 湛江 524088  
叶国玲 中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 农业农村部南海渔业资源环境科学观测实验站, 广东珠江口生态系统野外科学观测研究站, 广州 510300  
陈海刚 中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 农业农村部南海渔业资源环境科学观测实验站, 广东珠江口生态系统野外科学观测研究站, 广州 510300 hgchenes@163.com 
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中文摘要:
      为探究典型溴化阻燃剂六溴环十二烷(HBCD)和重金属 Cu2+对海水小球藻(Chlorella salina)的毒性作用,本研究测定HBCD和Cu2+单独和联合暴露96 h后小球藻的细胞密度、Chl a含量和叶绿素荧光的变化情况。结果表明:单独暴露下,HBCD处理组藻细胞密度与对照组相比显著下降,其中 500 μg·L-1 HBCD 处理组抑制率最高(32.99%),Chl a 含量和Fv/Fm则与对照组无显著差异;Cu2+处理组中,96 h-EC50分别为 637 μg·L-1( 细胞密度)和 541 μg·L-1( Chl a含量),Chl a含量对 Cu2+的敏感性高于细胞密度,海水小球藻的生长受到明显抑制且呈剂量-效应关系,1 260 μg·L-1和2 000 μg·L-1 Cu2+处理组中Fv/Fm分别显著降低24.24%和32.32%;HBCD和Cu2+联合暴露下,海水小球藻的细胞密度和Chl a含量的抑制率增高、叶绿素荧光参数Fv/FmΦPSⅡ比Cu2+单独暴露低,基于细胞密度计算的96 h EC10和EC50则随HBCD浓度的增加而显著降低。析因分析表明,HBCD和Cu2+联合暴露对海水小球藻的影响具有交互作用,HBCD 会增加 Cu2+对海水小球藻的毒性。研究表明,HBCD 对海水小球藻的毒性低,但低浓度HBCD与Cu2+联合暴露下Cu2+的毒性明显增强,环境中HBCD和Cu2+联合作用对海洋生态系统具有较大的潜在风险。
英文摘要:
      To explore the toxic effects of a typical brominated flame retardant hexabromocyclododecane(HBCD)and heavy metal Cu2+ , Chlorella salina was exposed to HBCD and Cu2+ treatments separately and in combination for 96 h, respectively. The cell density, chlorophyll a(Chl a)content, and chlorophyll fluorescence parameters(Fv / Fm and ΦPS Ⅱ)of C. salina were measured. The results showed that the cell density of C. salina in the HBCD treatment group decreased significantly compared with that in the control group; the inhibition rate of 500 μg·L-1 in the HBCD treatment group was the highest(32.99%), while the content of Chl a and Fv/Fm were not significantly different from those in the control group. In the Cu2+ treatment group, the 96 h-EC50 was 637 μg·L-1( cell density)and 541 μg· L-1(Chl a content), respectively. The sensitivity of Chl a content to Cu2+ was higher compared to cell density. Although significant inhibition and concentration-related effects were observed on the growth of C. salina in the Cu2+ treatment group, its growth in the Cu2+ treatment was decreased by 24.24% and 32.32%, in the presence of Fv/Fm concentrations of 1 260 μg·L-1 and 2 000 μg·L-1, respectively. Under the combined exposure of HBCD and Cu2+, the inhibition rate of cell density and Chl a content of C. salina increased, while the inhibition rate of Fv/Fm and ΦPSⅡ were lower than those of cells exposed to Cu2+ separately. Based on the cell density, the 96 h EC10 and EC50 values were calculated; these decreased significantly with the increase in HBCD concentration. Factorial analysis showed that the combined exposure of HBCD and Cu2+ had an interactive effect on C. salina and HBCD increased the toxicity of Cu2+ to C. salina. This study showed that HBCD exhibits low toxicity to C. salina, while the toxicity of Cu2+ was significantly enhanced with the combined exposure of low concentrations of HBCD and Cu2+. Therefore, the combined effect of HBCD and Cu2+ in the environment poses a great potential risk to marine ecosystems.
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