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| 藻菌共培养体系优势菌株筛选及沼液处理 |
| Screening of the dominant strains in the algae-bacteria symbiotic system and effects of biogas slurry treatment |
| 投稿时间：2018-03-19 |
| DOI：10.13254/j.jare.2018.0068 |
| 中文关键词: 小球藻，藻菌共培养，沼液废水，总氮，总磷，COD |
| 英文关键词: Chlorella vulgaris, algae-bacteria symbiotic system, biogas slury wastewater, total nitrogen, total phosphorus, COD |
| 基金项目:山东省农业重大应用技术创新项目（鲁财农指（2016）36号）；山东省现代农业产业技术体系建设专项资金项目（SDAIT-26-03）；烟台大学科技创新基金项目（YDZD1818） |
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| 中文摘要: |
| 本研究从小球藻处理沼液废水的体系中分离细菌，探究小球藻与细菌之间相互作用关系及藻菌体系对废水处理的效果。经多次平板划线从体系中共分离出5种菌株经16S rDNA基因测序，5种菌株分别与细菌Pseudomonas alcaliphila、Exiguobacterium、Bacillus amyloliquefaciens、Bacillus subtilis和Bacillus tequilensis高度相似。通过测定小球藻的叶绿素a的变化发现5种细菌均可促进小球藻生长，其中细菌Exiguobacterium对小球藻的促生效果最为明显。将几株单菌株分别和小球藻共同培养，考察共培养体系对沼液的净化效果，结果发现细菌Pseudomonas alcaliphila与小球藻共培养体系中沼液总磷（TP）下降到0.391 mg·L-1，去除率最高，达85%；细菌Exiguobacterium与小球藻共培养体系中沼液总氮（TN）和化学需氧量（COD）去除率分别为64.4%和72.3%，效果最优；小球藻纯培养体系和细菌纯培养体系对沼液中COD、TN、TP的去除率，均远低于细菌Pseudomonas alcaliphila和Exiguobacterium与小球藻构建的藻菌共培养体系。综合比较，藻菌共培养体系对污水处理效果优于各自的纯培养体系，在污水处理中具有很好的应用前景。 |
| 英文摘要: |
| Bacteria were separated from biogas slurry treated by Chlorella vulgaris, and then the interaction between Chlorella vulgaris and bacteria was explored and the effect of algae-bacteria symbiotic system on biogas slurry treatment was discussed. 5 bacteria were separated by flat-plate separation method and were identified by 16S rDNA sequencing technology. They were highly similar to the following bacteria:Pseudomonas alcaliphila, Exiguobacterium, Bacillus amyloliquefaciens, Bacillus subtilis and Bacillus tequilensis. By measuring changes in chlorophyll a of Chlorella vulgaris, it was found that 5 bacteria could promote the growth of chlorella and the effect of Exiguobacterium was most obvious. These single strain were co-cultured with Chlorella vulgaris separately and the purification effect of algae-bacteria symbiotic system on biogas slurry were investigated. The results showed that the consortium of Pseudomonas alcaliphila and Chlorella vulgaris could reduce TP to 0.391 mg·L-1, and its removal rate was the highest(85%). The consortium of Exiguobacterium and Chlorella vulgaris had the best treatment effect on TN and COD, and the removal rate was 64.4% and 72.3% respectively. The removal rates of COD, TN and TP by pure microalgae or pure axenic bacterium were much lower than that of the above two microalgae and bacteria co-culture systems. To summarize, algae-bacteria symbolic system has better effect on sewage treatment than pure culture system and has a good application prospect in sewage treatment. |
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