| 卢静,侯彬,郭楚玲.多环芳烃降解菌的细胞融合及降解性能研究[J].农业环境科学学报,2015,34(6):1134-1141. |
| 多环芳烃降解菌的细胞融合及降解性能研究 |
| Cell Fusion of PAHs-degrading Bacteria and Its Degradation Ability |
| 投稿时间：2015-01-22 |
| DOI：10.11654/jaes.2015.06.017 |
| 中文关键词: 菲 芘 融合菌 遗传标记 多环芳烃 |
| 英文关键词: phenanthrene pyrene fusant strain genetic marker PAHs |
| 基金项目:国家自然科学基金青年基金(41401355,41101291);山西省青年科技研究基金(2014021034-2,2015021053) |
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| 中文摘要: |
| 以菲降解菌--鞘氨醇单胞菌(Sphingomonas sp.)GY2B和芘降解菌--假单胞菌(Sphingomonas sp.)GP3A为研究对象, 对两株菌进行融合前的抗药性标记筛选, 融合后的菌株通过形态学及分子生物学进行分析鉴定, 并测定其对菲和芘的降解效果。结果表明, 筛选出GY2B的遗传标记为哌拉西林抗性(80 μg·mL-1),GP3A的遗传标记为头孢他啶抗性(80 μg·mL-1)或红霉素抗性(100~150 μg·mL-1).通过菲和芘的初步降解实验筛选出一株融合菌株F14, 通过平板菌落形态、扫描电镜(SEM)及PCR-RFLP分析鉴定F14是不同于亲本的菌株, 是GY2B 和GP3A 的融合子。融合菌株F14既可以降解菲又可以降解芘, 对初始浓度为100 mg·L-1的菲和芘的降解率分别为99%(24 h)和18%(10 d),降解能力和降解效果明显高于其亲本。 |
| 英文摘要: |
| In the present study, a phenethrene-degradation strain(Sphingomonas sp.) GY2B and a pyrene-degradation strain(Pseudomonas sp.) GP3A were used to obtain a fusant capable of degrading PAHs. The antibiotic resistance test was used to screen the genetic markers. Morphology and molecular biology were employed to identify fusants. Results showed that the genetic markers of Sphingomonas sp. GY2B was piperacillin(80 μg·mL-1)-resistant and Pseudomonas sp. GP3A was ceftazidime(80 μg·mL-1)- or erythromycin(100~150 μg·mL-1)-resistant, respectively. A fusant strain with the highest degradation of both phenanthrene and pyrene was selected and named F14. Tests by colony morphology, electron microscope, scanning electron microscope(SEM) and molecular technology(PCR-RFLP) indicated that the strain F14 was a fusant of GP3A and GY2B, and was different from its parents. F14 could degrade both phenanthrene and pyrene. It almost completely degraded phenanthrene(100 μg·mL-1) within 24 hours, much quicker than GY2B and other strains. F14 degraded 18% of pyrene(100 mg·L-1) within 10 days, still higher than GP3A. |
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