| 刘聪,许平平,王亚,郑燕恒,林巧云,唐皓,张春华,葛滢.不同氮磷浓度对蛋白核小球藻砷富集和转化的影响[J].农业环境科学学报,2016,35(9):1665-1671. |
| 不同氮磷浓度对蛋白核小球藻砷富集和转化的影响 |
| Effects of various nitrogen and phosphorus concentrations on arsenic accumulation and transformation in Chlorella pyrenoidesa |
| 投稿时间：2016-03-26 |
| DOI：10.11654/jaes.2016-0402 |
| 中文关键词: 蛋白核小球藻 氮 磷 砷 富集 转化 |
| 英文关键词: Chlorella pyrenoidesa nitrogen phosphorus arsenic accumulation transformation |
| 基金项目:国家自然科学基金项目（41371468，31400450） |
| 作者 | 单位 | E-mail | | 刘聪 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 许平平 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 王亚 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 郑燕恒 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 林巧云 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 唐皓 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | | | 张春华 | 南京农业大学生命科学实验中心, 元素与生命科学示范实验室, 南京 210095 | | | 葛滢 | 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095 | yingge711@njau.edu.cn |
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| 中文摘要: |
| 氮（N）、磷（P）是影响蛋白核小球藻生长的重要因素，通过改变培养液中N、P的浓度，可能实现对蛋白核小球藻富集砷（As）进行调控。为探讨N、P浓度对这种微藻吸收As的影响是否与其生长变化有关，采用室内培养实验，首先研究不同N、P浓度对蛋白核小球藻生长的影响；进而选择不影响小球藻生长的N（247、24.7 mg·L-1）、P（6、0.6 mg·L-1）浓度组合，设置0.8、8 mg·L-1的亚砷酸盐（As3+）和砷酸盐（As5+）处理3 d，研究N、P浓度对小球藻As富集和转化的影响。结果表明，当P浓度为6 mg·L-1时，N浓度降低到24.7 mg·L-1不会影响小球藻对As3+和As5+的富集及其胞内As形态的转化；而当N浓度为247 mg·L-1时，P浓度降低到0.6 mg·L-1则会显著增加小球藻对As3+和As5+的吸收和富集，藻细胞内As5+还原、甲基化和外排也显著增强。因此，在不影响小球藻细胞生长的条件下，P对其As富集和转化过程的影响比N更为显著。 |
| 英文摘要: |
| Nitrogen(N) and phosphorus(P) are important factors controlling the growth of microalgae such as Chlorella pyrenoidesa. The accumulation of arsenic(As) by this alga may be regulated through adjusting N and P concentrations in the culture. However, it is unclear whether the effects of N and P on the As uptake are related to microalgal growth changes. Therefore, we first studied the impacts of N and P concentrations on the growth of C. pyrenoidesa, and then chosen N(247 mg·L-1, 24.7 mg·L-1) and P(6 mg·L-1, 0.6 mg·L-1) concentrations that had no effects on the algal growth, to investigate their effects on the As metabolism in this algae under exposure of 0.8 mg·L-1 and 8 mg·L-1 As3+ and As5+. Results showed that the algal As accumulation and transformation were not affected when P was 6 mg·L-1 and N was decreased from 247 mg·L-1 to 24.7 mg·L-1. However, these processes were significantly impacted when N was 247 mg·L-1 and P was decreased from 6 mg·L-1 to 0.6 mg·L-1. The decrease in P supply also promoted the reduction, methylation and excretion of As in C. pyrenoidesa. These results indicate that, when N and P have no effects on the algal growth, P influences the As accumulation and transformation greater than N does. This study would provide a theoretical basis for elucidating the regulatory mechanisms of As metabolism and enhancing the efficiency of As pollution remediation in water. |
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