文章摘要
胡芳雨,孟凡波,张闻,高永超,郑立稳.黑麦草根系分泌物氨基酸组分对PAHs胁迫的响应[J].农业环境科学学报,2020,39(9):1937-1945.
黑麦草根系分泌物氨基酸组分对PAHs胁迫的响应
Response of amino acids in ryegrass root exudates to polycyclic aromatic hydrocarbon stress
投稿时间:2020-04-13  
DOI:10.11654/jaes.2020-0414
中文关键词: 黑麦草  根系分泌物  氨基酸  多环芳烃
英文关键词: ryegrass  root exudates  amino acid  polycyclic aromatic hydrocarbons(PAHs)
基金项目:国家自然科学基金项目(41601331);山东省重点研发计划项目(2019GSF109102);山东省自然科学基金项目(ZR2019BD012)
作者单位E-mail
胡芳雨 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103  
孟凡波 济南市环境研究院, 济南 250102  
张闻 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 zw-sunshine@163.com 
高永超 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103  
郑立稳 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103  
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中文摘要:
      为探索黑麦草根系分泌物中的氨基酸对多环芳烃(PAHs)胁迫的响应,通过水培试验,研究了在不同浓度的菲、芘和苯并(a)芘胁迫下,黑麦草根系分泌物中氨基酸的变化。结果表明:无PAHs胁迫时,黑麦草根系分泌物的氨基酸总量为(84.2±10.3)μg·g-1,共检出10种氨基酸,其中缬氨酸和谷氨酸浓度相对较高,各占氨基酸总量的43%和24%。PAHs胁迫使黑麦草生物量有不同程度的增加,并影响根系分泌的氨基酸总量及组分构成,其胁迫效应因PAHs种类和浓度而异。对于氨基酸总量,菲和低浓度苯并(a)芘均起抑制作用,且高浓度菲比低浓度抑制效应更强;其他处理无显著影响。对于氨基酸组分构成,菲使黑麦草不再分泌酪氨酸并抑制缬氨酸的分泌,高浓度时还对谷氨酸分泌起抑制作用。芘的存在使黑麦草开始分泌异亮氨酸及亮氨酸,高浓度时还可促使蛋氨酸分泌,低浓度时则抑制了缬氨酸的分泌且使黑麦草不再分泌组氨酸。苯并(a)芘使黑麦草开始分泌异亮氨酸和亮氨酸,而不再分泌酪氨酸,并抑制缬氨酸的分泌,此外,高浓度时还使黑麦草开始分泌蛋氨酸,低浓度时则使黑麦草不再分泌组氨酸。
英文摘要:
      In this study, a hydroponics experiment was conducted to investigate the effects of different concentrations of phenanthrene (PHE), pyrene(PYR), and benzo(a)pyrene(BAP)on the amino acids in ryegrass root exudates. A total of(84.2±10.3)μg·g-1 of amino acids in ryegrass root exudates was identified in the control group. In total, ten amino acids were detected, of which valine and glutamic acid accounted for 43% and 24%, respectively. The biomass of ryegrass increased to different extents after exposure to polycyclic aromatic hydrocarbons(PAHs), which showed different effects on the total concentration and composition of the amino acids. The total concentration of amino acids in the root exudates decreased in PHE and BAP-L groups and an increased inhibition effect was observed with the increase in PHE concentration. Other treatments did not result in significant differences. The exudation of tyrosine was not detected under PHE stress while the exudation of valine was inhibited. At the same time, the exudation of glutamic acid was inhibited by PHE-H stress. However, the exudation of isoleucine and leucine were promoted by PYR stress. In addition, the exudation of methionine was promoted by PYR-H stress while the exudation of valine was inhibited and that of histidine was stopped by PYR-L stress. The exudation of isoleucine and leucine were initiated by BAP stress, which stopped the exudation of tyrosine and inhibited the exudation of valine. Furthermore, the exudation of methionine was promoted by BAP-H stress and that of histidine was stopped by BAP-L stress. Exploration of the effect of PAHs on amino acids in ryegrass root exudation can provide insights into the mechanism of phytoremediation in contaminated soils.
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