| 张伟,窦森.纤维素降解过程中链霉菌菌体及其碱提取物组分研究[J].农业环境科学学报,2014,33(12):2412-2419. |
| 纤维素降解过程中链霉菌菌体及其碱提取物组分研究 |
| Chemical Characterization of Cellulose-degrading Streptomyces and Its Alkaline Extraction Fraction During Cellulose Degradation |
| 投稿时间：2014-04-12 |
| DOI：10.11654/jaes.2014.12.019 |
| 中文关键词: 链霉菌 纤维素 碱提取物组分 结构特征 |
| 英文关键词: Streptomyces sp. cellulose alkaline extraction component structural characteristics |
| 基金项目:国家自然基金重点项目(40771215);国家重点基础研究发展计划(2010CB428804-2);国家高技术研究发展计划(2011AA050105);中国科学院知识创新工程方向项目(KZCX2-EW-QN606) |
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| 中文摘要: |
| 针对链霉菌降解纤维素后是否能形成腐殖质及其碱提取物组分是否为腐殖质组分这一微生物利用问题,采用液体摇床振荡培养实验,获得链霉菌降解纤维素形成的菌体,利用元素组成、差热分析和红外光谱法等现代仪器分析手段,初步研究了菌体的化学结构和碱提取物组分碳的分配状况.结果表明:随着培养时间的延长,培养后期(60 d)的链霉菌菌体产率显著增加;链霉菌菌体在化学结构上相似,与黑土胡敏酸(HA)相比,菌体的结晶度较低,芳香性较弱,热稳定性较强,脂肪碳链和含氧官能团含量较高;链霉菌经纤维素作用后形成的菌体,与黑土碱提取物(胡敏酸、富里酸)相比,"水溶性组分"较多,"碱溶组分"(类似于胡敏酸和富里酸的总和)较少,富含"碱溶酸不溶组分(类似于胡敏酸)"的物质增多,"水溶性组分"和"碱溶酸不溶组分"与纤维素和链霉菌的共同作用有关.以上结果表明,纤维素培养过程中链霉菌菌体与真正的黑土胡敏酸(HA)是有差别的. |
| 英文摘要: |
| Streptomyces are Gram-positive filamentous bacteria that live in the superficial layer of soil where they can degrade cellulose. They play important roles in producing the currently known antibiotics and many other bioactive molecules useful to human health and agriculture. However, little is known about the characteristics of mycelia and alkaline extraction of Streptomyces sp. during cellulose degradation. In the present study, sodium carboxymethyl cellulose(CMC) was used as a unique carbon source for the isolated strain Streptomyces sp. F in the liquid culture condition during the 60 day period. Carbon distribution, elemental compositions, thermostability and infrared structure of the alkali extraction of the harvested cells were further explored. The results showed that sodium carboxymethyl cellulose could be used as a unique carbon and energy source for the growth of strain Streptomyces sp. F. There was a significant increase in the production rate of harvested cells(α=0.05) during incubation. At the end of incubation(60 d), this production rate reached at 7.29%. Though the elemental composition was similar between the "mycelia" of harvested cells at different culture time and the humic acid(HA) of black soil, the mycelia showed different characteristics from these of the soil HA, with lower degree of crystallinity, greater thermal stability, weaker aromaticity, and higher aliphatic carbon chain and oxygen-containing functional group content. Compared with HA from black soil, Streptomyces had more "water-soluble component" groups and "alkali-soluble acid-insoluble groups(the equivalent of humic acid)" but less "alkali-soluble component groups(the equivalent of humic and fulvic acid)". These results suggest that both the "water-soluble component" and "alkali-soluble acid-insoluble fraction " were related with the breakdown of sodium carboxymethyl cellulose and the biological synthesis in "mycelia", and that the "alkali-soluble acid-insoluble groups" characteristics of the "mycelia" was distinct from the humic acid of the real black soil(HA). |
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