镉急性染毒对中华稻蝗精卵巢小分子热休克蛋白基因表达的影响

寇利花; 吴海花; 刘耀明; 张育平; 张建珍; 郭亚平; 马恩波

文章摘要

寇利花,吴海花,刘耀明,张育平,张建珍,郭亚平,马恩波.镉急性染毒对中华稻蝗精卵巢小分子热休克蛋白基因表达的影响[J].农业环境科学学报,2015,34(1):7-14.

镉急性染毒对中华稻蝗精卵巢小分子热休克蛋白基因表达的影响

Expression Analysis of OcsHSP Genes in Testis and Ovary of Oxya chinensis Exposed to Acute Cadmium

投稿时间：2014-07-14

DOI：10.11654/jaes.2015.01.002

中文关键词: 中华稻蝗小分子热休克蛋白镉实时定量PCR 精巢卵巢

英文关键词: Oxya chinensis small heat shock protein Cd real-time quantitative PCR testis ovary

基金项目:国家自然科学基金项目(31071980,31172161)

作者	单位	E-mail
寇利花	山西大学应用生物学研究所, 太原 030006
吴海花	山西大学应用生物学研究所, 太原 030006
刘耀明	山西大学应用生物学研究所, 太原 030006
张育平	太原师范学院生物系, 太原 030031
张建珍	山西大学应用生物学研究所, 太原 030006
郭亚平	山西大学生命科学学院, 太原 030006
马恩波	山西大学应用生物学研究所, 太原 030006	maenbo2003@sxu.edu.cn

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中文摘要:

小分子热休克蛋白(sHSPs)能够被环境胁迫诱导,采用不同浓度氯化镉(CdCl2)溶液对中华稻蝗5龄若虫进行急性染毒, 利用Real-time PCR技术对5个小分子热休克蛋白基因OcHSP19.1、OcHSP19.8、OcHSP20.4、OcHSP20.7和OcHSP21.1在精巢和卵巢的表达进行了分析.结果表明:在精巢内,OcHSP19.1和OcHSP19.8基因经0.16 μg·μL^-1 Cd²⁺ 处理,其在不同处理时间的表达水平与对照组相比均显著升高;经0.32 μg·μL^-1 Cd²⁺ 处理,其在2 h表达水平最高,之后显著降低;经0.48 μg·μL^-1 Cd²⁺ 处理,OcHSP19.1基因在2 h和6 h的表达量均高于对照组,在12 h的表达量低于对照组,OcHSP19.8基因在2 h的表达量高于对照组,在6 h的表达量低于对照组,之后二者表达量都显著升高,在36 h表达量最高,分别为对照组的6.3倍和5.4倍.在卵巢内,OcHSP19.1和OcHSP21.1基因经0.16 μg·μL^-1和0.48 μg·μL^-1 Cd²⁺ 处理,其在不同处理时间的表达水平与对照组相比无显著差异;经0.32 μg·μL^-1 Cd²⁺ 处理,OcHSP19.1基因在6 h和12 h的表达水平高于对照组,但在24 h低于对照组,OcHSP21.1基因在2、6、12 h和24 h的表达水平与对照组相比均显著降低,但二者都在36 h表达量最高,分别为对照组的4.8倍和4.6倍;经0.16 μg·μL^-1和0.48 μg·μL^-1 Cd²⁺ 处理,OcHSP20.7基因在2 h表达水平高于对照组,经0.32 μg·μL^-1 Cd²⁺ 处理,该基因的表达水平随着时间的延长出现先升高后降低的趋势.OcHSP21.1基因在精巢内和OcHSP20.4基因在卵巢内的表达水平与对照组相比无显著差异.研究结果显示,Cd急性诱导中华稻蝗5个OcsHSPs基因在精巢和卵巢的表达模式不同,其表达量与Cd浓度和作用时间相关.

英文摘要:

Small heat shock proteins(sHSPs) can be induced by environmental stresses. In the present study, we analyzed the mRNA expression patterns of OcHSP19.1, OcHSP19.8, OcHSP20.4, OcHSP20.7, and OcHSP21.1 genes in the testis and ovary of the fifth-instar nymphs of Oxya chinensis exposed to acute cadmium(Cd) toxicity using real-time quantitative PCR. In the testis, 0.16 μg·μL^-1 Cd²⁺ induced the expression of OcHSP19.1 and OcHSP19.8 genes in different treatment times. However, 0.32 μg·μL^-1 Cd²⁺ did not cause significant changes of their expression as compared with the control. When treated with 0.48 μg·μL^-1 Cd²⁺, the expression levels of OcHSP19.1 were higher at 2 h and 6 h but lower at 12 h, and those of OcHSP19.8 were higher at 2 h but lower at 6 h, in comparison with the control. Both reached the highest level at 36 h. In the ovary, 0.16 μg·μL^-1 and 0.48 μg·μL^-1 Cd²⁺ did not significantly change OcHSP19.1 and OcHSP21.1 genes in different treatment times, but induced the expression of OcHSP20.7 gene at 2 h, compared with the control. In the treatment by 0.32 μg·μL^-1 Cd²⁺, the expression levels of OcHSP19.1 gene were higher at 6 h and 12 h but lower at 24 h, and the expression levels of OcHSP21.1 were lower at 2 h, 6 h, 12 h, and 24 h, as compared with the control. Both genes reached the highest level at 36 h. The expression levels of OcHSP20.7 gene first increased then declined significantly. The expression levels of OcHSP21.1 gene in the testis and OcHSP20.4 gene in the ovary did not significantly differ from the controls. The results show that the expression patterns of the five OcsHSP genes are related to the Cd concentrations and treatment times.

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