| 田向荣,吴昊,李菁,李朝阳.Cd、Pb复合胁迫下湿地匍灯藓(Plagiomnium acutum)重金属累积效应及抗氧化系统响应[J].农业环境科学学报,2015,34(5):844-851. |
| Cd、Pb复合胁迫下湿地匍灯藓(Plagiomnium acutum)重金属累积效应及抗氧化系统响应 |
| Heavy Metal Accumulation and Antioxidative System Responses of Plagiomnium acutum Under Combined Cadmium and Lead Stresses |
| 投稿时间：2014-11-11 |
| DOI：10.11654/jaes.2015.05.005 |
| 中文关键词: 湿地匍灯藓(Plagiomnium acutum) Cd、Pb复合胁迫 累积效应 抗氧化系统 响应 |
| 英文关键词: Plagiomnium acutum combined cadmium and lead stress accumulation antioxidative system response |
| 基金项目:国家自然科学基金项目(31360062);湖南省重点学科建设项目(JSU071312Z01) |
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| 中文摘要: |
| 采用浸没培养方法研究了不同浓度Cd、Pb复合胁迫下湿地匍灯藓(Plagiomnium acutum)重金属的累积效应及抗氧化系统的响应。结果显示:复合胁迫下Pb显着抑制湿地匍灯藓对Cd的吸收,与低 Pb 水平(20 mg·L-1)相比(对照无检出值),在低 Cd(2 mg·L-1)处理时,随Pb 处理浓度增加,藓体内 Cd 累积量分别降低了30.1%和 78.4%;在高 Cd(50 mg·L-1)水平处理时,其降幅则分别达到了 35.0%和 86.9%.复合胁迫引起湿地匍灯藓脂质过氧化终产物丙二醛(MDA)含量随胁迫程度加大而持续升高,当复合胁迫浓度达到最大时,MDA含量也达到了最大,与对照相比显着增加7162%.与此同时,超氧化物歧化酶(SOD)活性和脯氨酸含量也呈现了应激性增加,且均在最高复合胁迫浓度处理时达到最大,与对照相比分别增加237.4%和2049%,而过氧化氢酶(CAT)和过氧化物酶(POD)活性则持续降低。基于此,可以认为:Cd、Pb复合胁迫下,Pb对Cd的累积效应有明显的抑制作用,且这种抑制作用有浓度依赖性;Cd、Pb复合胁迫所致细胞氧化损伤的主要来源为过氧化物的清除能力低下所致过氧化物积累。 |
| 英文摘要: |
| Moss, as a pioneer plant of the whole ecosystem, possesses several special physiological characteristics to adapt the severe environment, such as extreme cold and heavy metal contamination. Here, a submerging culture experiment was designed to explore heavy metal accumulation and antioxidative responses of Plagiomnium acutum under combined lead and cadmium stresses. The presence of lead obviously inhibited absorption of cadmium under combined stresses. Compared with 20 mg·L-1 lead treatment, cadmium absorption were reduced by 30.1% and 78.4% under low cadmium treatment(2 mg·L-1), and by 35.0% and 86.9% under high cadmium treatment(50 mg·L-1), by 50 mg·L-1 and 100 mg·L-1 lead, respectively. The content of lipid peroxidation final product, malondialdehyde(MDA), increased with elevated combined stresses. The highest MDA level was 7162% over the control. Superoxide dismutase(SOD) activity and proline content were the highest under the greatest combined stress, which were respectively 237.4% and 2049% over the control. The SOD activities(R2=1, 0.935, 0.481) and proline content(R2=0.955, 0.927 and 0.999) was mainly related to lead. On the contrary, activities of peroxidase(R2=0.451, 0.997 and 0.976) and catalase(R2=0.726, 0.850 and 0.725) were negatively impacted mainly by cadmium under combined stresses. In summary, (1) lead can inhibit cadmium absorption in a dose-effect manner; (2) the main toxicological reaction should be cellular oxidative damage caused by cadmium; and (3) the preliminary source of cell oxidative damage in Plagiomnium acutum under combined stresses is the accumulation of peroxide from weakened ability of peroxide scavenging. |
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