| 李定心,樊霆,唐子阳,王振,陈海燕,鲁洪娟,张国漪,叶文玲,张伟.Cu(Ⅱ)胁迫下黑曲霉TL-F2谷胱甘肽系统的响应[J].农业环境科学学报,2015,34(5):852-858. |
| Cu(Ⅱ)胁迫下黑曲霉TL-F2谷胱甘肽系统的响应 |
| Responses of Glutathione System of Aspergillus niger TL-F2 to Cu(Ⅱ) Stress |
| 投稿时间：2014-12-12 |
| DOI：10.11654/jaes.2015.05.006 |
| 中文关键词: 黑曲霉 抗氧化 谷胱甘肽系统 铜 |
| 英文关键词: Aspergillus niger TL-F2 anti-oxidation glutathione system copper |
| 基金项目:国家自然科学基金项目(41101485);安徽省国土资源科技项目(2012-K-12);安徽农业大学稳定和引进人才科研资助项目;安徽省级大学生创新训练项目(AH201310364024) |
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| 中文摘要: |
| 以矿区筛选抗性黑曲霉(Aspergillus niger TL-F2)为材料,研究不同浓度(50、100、150、200、250、300 mg·L-1)Cu(Ⅱ)对不同生长阶段黑曲霉谷胱甘肽系统(GSH/GPx)的影响。结果显示:随着Cu(Ⅱ)浓度增大,A.niger TL-F2生长量、可溶性蛋白含量呈降低趋势,丙二醛(MDA)含量则增加;谷胱甘肽系统中还原型谷胱甘肽(GSH)含量呈现上升趋势,而氧化型谷胱甘肽(GSSG)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽还原酶(GR)活力则呈现先增后降趋势;在金属浓度为150 mg·L-1时GSSG含量达到最大为365.68 μmol·L-1,GPx和GR活力分别在金属浓度为100 mg·L-1时达到峰值,GSH/GSSG比值也随Cu(Ⅱ)浓度和处理时间的增加呈下降趋势。结果表明:在Cu(Ⅱ)作用下A.niger TL-F2菌体内GSH/GPx系统产生氧化应激反应,通过调节GSH、GSSG、GPx和GR的增减来抵御过量活性氧(ROS)胁迫;GSH/GSSG比值也可直观地反映Cu(Ⅱ)对A.niger TL-F2毒性大小。 |
| 英文摘要: |
| Aspergillus niger is widely used in removal of heavy metals from wastewater and polluted soil. A strain resistant to copper ions of high concentrations, named TL-F2, was isolated from a copper tailing. The effects of initial Cu(Ⅱ) concentrations(50, 100, 150, 200, 250, 300 mg·L-1) on growth and antioxidant system of Aspergillus niger TL-F2(A. niger TL-F2) were investigated at different growth stages. With increasing Cu(Ⅱ) concentrations, both biomass and soluble protein content of A. niger TL-F2 decreased, but malondialdehyde(MDA)content in A. niger TL-F2 increased. Compared with the control, the activity of GSH increased at all time. However, the content of oxidized glutathione(GSSG) and the activities of GPx and glutathione reductase(GR) displayed “initial increase and then decrease” over Cu(Ⅱ)concentrations and culture time. The concentration of GSSG was the highest of 365.68 μmol·L-1 at Cu(Ⅱ) concentration of 150 mg·L-1, while the activities of GPx and GR reached their maximum at Cu(Ⅱ) concentration of 100 mg·L-1. The ratio of GSH/GSSG decreased with increasing Cu(Ⅱ) concentrations and culture time. In summary, the GSH/GPx system in A.niger TL-F2 produced oxidative stresses to resist the excessive reactive oxygen species(ROS) via regulating the content or activities of GSH, GSSG, GPx and GR, under Cu(Ⅱ) stresses. It suggests that GSH/GPx system plays an important role in the antioxidant system in A. niger TL-F2 exposed to Cu(Ⅱ), and the ratio of GSH/GSSG values can also be an indicator of Cu(Ⅱ) toxicity to A. niger TL-F2. |
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