| 蒋婷,姜贝贝,潘远智,谭淼,张梦琪,杨亚男,刘柿良.不同形态氮素对龙葵镉毒害的缓解效应[J].农业环境科学学报,2015,34(6):1060-1068. |
| 不同形态氮素对龙葵镉毒害的缓解效应 |
| Mitigation of Different Forms of Exogenous Nitrogen on Cd Toxicity to Solanum nigrum |
| 投稿时间：2015-01-07 |
| DOI：10.11654/jaes.2015.06.007 |
| 中文关键词: 氮素 龙葵 镉胁迫 缓解效应 抗坏血酸-谷胱甘肽循环 |
| 英文关键词: nitrogen Solanum nigrum cadmium stress mitigation AsA-GSH cycle |
| 基金项目:四川农业大学大学生创新性实验计划(1310626068);四川省大学生创新创业训练计划(201410626051) |
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| 中文摘要: |
| 为探讨不同形态氮素对龙葵(Solanum nigrum)镉毒害的缓解效应, 采用温室盆栽试验, 研究了外源添加不同形态氮素(NO3--N[NaNO3]、NH4+-N[(NH4)2SO4]和NH4+-N+NO3--N [NH4NO3]),对40 mg·kg-1 Cd胁迫下龙葵生长的影响。试验结果表明, 40 mg·kg-1 Cd显着抑制了龙葵的生长;外源添加一定浓度的不同形态氮素可显着缓解龙葵的Cd胁迫:NH4+-N对龙葵地上部生物量的增产效果优于NO3--N和NH4+-N+NO3--N;不同浓度NO3--N的施加显着降低叶片CAT和POD活性, SOD活性呈先升后降趋势;CAT活性随着添加NH4+-N浓度的增加先升后降, POD活性逐渐上升, SOD活性逐渐下降;叶片CAT与SOD活性随着NH4+-N+NO3--N添加浓度的增加逐渐降低至稳定, 而POD活性则先升后降;叶片超氧阴离子产生速率、H2O2和MDA含量显着低于对照;NH4+-N和NH4+-N+NO3--N处理下的GR活性降低, 添加NO3--N的则先上升后下降。AsA含量和APX活性则较无外源氮素处理的龙葵升高, 较低浓度的NO3--N处理下GSH含量呈现随其浓度的增加逐渐升高的趋势。NH4+-N对龙葵镉毒害的缓解效应优于其他两种处理方式。 |
| 英文摘要: |
| Cadmium(Cd) pollution poses a serious risk to human health. Solanum nigrum is a Cd-hyperaccumulator. However, Cd absorption by Solanum nigrum may be affected by different forms of exogenous nitrogen. Here we did an experiment in greenhouse to investigate the effects of different forms of exogenous nitrogen(NO3--N[NaNO3]、NH4+-N[(NH4)2SO4] and NH4+-N+NO3--N [NH4NO3]) on Solanum nigrum seedlings exposed to 40 mg·kg-1 Cd in soil. At certain concentrations, different forms of exogenous nitrogen significantly relieved the Cd stress on Solanum nigrum treatment. Under Cd stress, the CAT and POD activities of Solanum nigrum leaves were significantly reduced, but SOD activity increased at first and then reduced by different concentrations of NO3--N. Exogenous NH4+-N increased CAT activity at first and reduced then; however, POD activity was gradually increased but SOD activity reduced by NH4+-N. Applying NH4+-N+NO3--N increased CAT and SOD activities, while led to a rise and then reduction in POD activity. Superoxide anion production rate, GR activity, H2O2 and MDA content of Solanum nigrum leaves under Cd stresses were significantly lower in exogenous nitrogen treatment than in no-nitrogen treatment. Exogenous nitrogen increased AsA content and APX activity, compared with those of no exogenous nitrogen treatment, Low concentrations of NO3--N increased GSH content. The experimental results show that NH4+-N is more effective in mitigating Cd stress of Solanum nigrum than others. |
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