蚯蚓(Eisenia foetida)对水稻土中Cd的富集及其氧化应激

陈娴; 王晓蓉; 季荣

文章摘要

陈娴,王晓蓉,季荣.蚯蚓(Eisenia foetida)对水稻土中Cd的富集及其氧化应激[J].农业环境科学学报,2015,34(8):1464-1469.

蚯蚓(Eisenia foetida)对水稻土中Cd的富集及其氧化应激

Bioaccumulation and Oxidative Stresses of Cadmium in Earthworm Eisenia Foetida in a Paddy Soil

投稿时间：2015-04-11

DOI：10.11654/jaes.2015.08.005

中文关键词: 土壤镉蚯蚓富集氧化应激

英文关键词: soil cadmium earthworm bioaccumulation oxidative stress

基金项目:国家自然科学基金重点项目(21237001);江苏理工学院青年科研基金项目(KYY13024)

作者	单位	E-mail
陈娴	江苏理工学院化学与环境工程学院, 江苏常州 213001 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210023
王晓蓉	污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210023	ekxr@nju.edu.cn
季荣	污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210023

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中文摘要:

以赤子爱胜蚓(Eisenia foetida)为模式生物,研究其暴露于不同外源Cd浓度(0～10 mg·kg^-1)水稻土中14 d后Cd等重金属在蚯蚓体内的富集,以及活性氧(ROS)、抗氧化酶和氧化损伤蛋白等生化指标对Cd胁迫的响应。结果表明,与其他金属相比,蚯蚓更易吸收和富集Cd,富集系数(BSAF)可达14.68,且外源Cd浓度的增加能显著抑制(P< 0.05)蚯蚓对Ni、Cu和Zn的富集。采用饱和模型对应用梯度扩散薄膜技术(DGT)测得的土壤有效态Cd和蚯蚓体内Cd含量进行拟合,结果显示良好的相关性(r²=0.99)。Cd胁迫能诱导蚯蚓体内产生活性氧(ROS)并导致蛋白质氧化损伤,但超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽硫转移酶(GST)活性呈现先降后升的"U"型剂量效应。通过比较可知,ROS和蛋白羰基(PCO)可作为土壤Cd污染早期诊断的生物标志物,水稻土中Cd对蚯蚓产生早期伤害可能的阈值范围为0.13~0.21 μg·L^-11 DGT-Cd。

英文摘要:

Ecological risk assessment and early diagnosis of soil heavy metal pollution are critical for soil environmental sustainability. In this study, earthworms(Eisenia foetida) were exposed to a paddy soil spiked with Cd at different concentrations(0~10 mg·kg^-1 dry soil) for 14 days. The bioaccumulation of Cd and other metals and the biochemical[active oxygen species(ROS), activities of anti-oxidative enzymes and oxidative damage proteins)] responses to Cd exposure of earthworms were determined. Cadmium had higher bioaccumulation in earthworms than other metals did, with a biota-to-soil accumulation factor(BSAF) of 14.86 for Cd. Cadmium at higher concentrations significantly inhibited bioaccumulation of Ni, Cu, and Zn(P< 0.05). Soil bioavailable Cd concentrations determined by Diffusive Gradient in Thin film technique(DGT) and earthworm Cd concentrations fit the Saturation Model well. ROS and protein carbonyl(PCO) were induced significantly(P< 0.01) by Cd additions, whereas the activities of superoxide dismutase(SOD), catalase(CAT), and glutathione S-transferase(GST) decreased initially but increased then, showing a U-shaped dose-response. Based on the present results, ROS and PCO were selected as biomarkers and the potential toxicity thresholds to earthworms were 0.13~0.21 μg·L^-1 DGT-Cd.

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