| 付倩,刘聪,赖金龙,吴国,陶宗娅,王昆艳.锶对蚕豆根尖细胞的遗传毒性效应[J].农业环境科学学报,2015,34(9):1646-1652. |
| 锶对蚕豆根尖细胞的遗传毒性效应 |
| Genotoxic Effects of Strontium on Root Tip Cells of Vicia faba L. |
| 投稿时间：2015-04-09 |
| DOI：10.11654/jaes.2015.09.003 |
| 中文关键词: 锶 DNA损伤 微核 彗星实验 蚕豆 |
| 英文关键词: strontium DNA damage micronucleus comet assays Vicia faba L. |
| 基金项目:四川省教育厅重点课题(14ZA0030);四川省教育厅大学生创新创业训练计划项目(201410636040);四川省生物质改性材料工程技术研究中心(西南科技大学)开放课题(12zxbk03);四川师范大学校级青年课题(14qn07);四川师范大学实验技术课题(SYJS2014-07) |
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| 中文摘要: |
| 为探讨Sr2+污染对蚕豆根尖细胞的生态毒性效应和对植物生长影响的毒理机制,以蚕豆为试材,通过急性毒性实验、微核实验和彗星实验,以ρ(Sr2+)0.01 mmol·L-1处理为CK,分析了Sr2+处理浓度[ρ(Sr2+)]为0.05~10 mmol·L-1对蚕豆根(芽)生长、干重、根尖细胞染色体结构及根尖细胞DNA断裂损伤的影响。结果显示,Sr2+对蚕豆的根尖细胞的生态毒性效应具有双重性。当ρ(Sr2+)<0.05 mmol·L-1时可促进蚕豆根、芽生长;ρ(Sr2+)>0.25 mmol·L-1时,蚕豆发芽率下降了17.93%~36.32%,根茎、生物量及总生物量分别降低了42.15%、48.69% 和46.75%。同时,Sr2+抑制了蚕豆根尖细胞有丝分裂,促进DNA链发生断裂,导致有丝分裂指数(MI)降低,出现染色体断片和微核,其微核率(MCN)是CK的200%~400%;彗星尾长(TL)、尾部DNA含量(TD)和彗星尾矩(TM)也显著高于CK(P<0.05),表明DNA出现了明显的断裂损伤。 |
| 英文摘要: |
| Strontium(Sr2+) in the environment easily enters plants and poses health risks to human via food pathway. This study was performed to investigate the genotoxic effects of Sr2+ on the root tip cells of broad bean(Vicia faba L.), a Sr2+ high-accumulating and sensitive plant, through acute toxicity test, micronucleus assay, and comet assay. The germination rate, seedling dry weight, chromosome breakage, and DNA damage of broad bean were examined at different Sr2+ concentrations [ρ(Sr2+)0.05~10 mmol·L-1] for 24 h, 48 h, and 72 h, with ρ(Sr2+) 0.01 mmol·L-1 as control(CK). Low concentrations of Sr2+ [ρ(Sr2+)<0.05 mmol·L-1] promoted elongation of the root and shoot of broad bean, but the germination rates were reduced by up to 17.93%~36.32% by ρ(Sr2+)> 0.25 mmol·L-1. As Sr2+ concentrations were ρ(Sr2+) 0.25~10 mmol·L-1, the average biomass and the mitotic index(MI) of the plant decreased, while the chromosomal aberration frequency(CAF), the micronucleus frequency(MCN) and the three parameter values of the comet assay(tail length, tail DNA%, and tail moment) all increased. In conclusion, Sr2+ has a dual effect on root tip cells, depending upon its concentration levels. The toxicity mechanism of Sr2+ on broad bean is structural damages of DNA in root tip cells. |
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