| 王京文,李丹,柳俊,侯昌萍,张奇春.耐镉菌株对土壤镉形态及土壤微生物群落结构的影响[J].农业环境科学学报,2015,34(9):1693-1699. |
| 耐镉菌株对土壤镉形态及土壤微生物群落结构的影响 |
| Effects of Cadmium Tolerant Bacteria on Soil Cadmium Forms and Microbial Community Structure |
| 投稿时间：2015-04-07 |
| DOI：10.11654/jaes.2015.09.010 |
| 中文关键词: 土壤 镉形态分级 耐镉菌 磷脂脂肪酸 微生物群落结构 |
| 英文关键词: soil cadmium fractionation cadmium tolerant bacteria phospholipid fatty acid(PLFA) microbial community structure |
| 基金项目:杭州市科技局项目(20140533B10);国家自然科学基金青年基金项目(J20140634) |
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| 中文摘要: |
| 通过传统的分离方法和室内培养实验,从污染土壤中筛选和分离耐镉菌株并分析耐镉菌株对土壤镉形态及微生物群落结构的影响。从浙江省富阳市电镀厂污染土壤中分离得到具有强活化作用的耐镉菌株m6,形态学观察和16S rDNA序列分析可知,m6与Arthrobacter的16S rDNA序列具有99%的同源性,其在系统发育地位上属于Bacteria,Actinobacteria,Actinobacteridae,Actinomycetales,Micrococcineae,Arthrobacter。研究表明耐镉菌株m6可使土壤中水溶态镉增加2倍多,可交换态镉增加16%。Biolog-ECO法的结果表明,接种耐镉菌株培养后土壤的AWCD值提高14%~146%,而且土壤微生物对31种碳源中的糖及衍生物、胺类碳源和多聚物类利用程度增加;磷脂脂肪酸法(PLFA)分析结果表明,与不接种菌株m6相比,接种土壤的脂肪酸总量增加了23.65%,代表真菌的特征脂肪酸(如18:2ω6,9、16:1ω5c、18:1ω9c)明显增加,但土壤的G-/G+比值与对照相比降低38.6%,而且土壤中代表放线菌的特征脂肪酸(如l0Me 16:0、l0Me 17:0和10Me 18:0)的多样性有所降低。 |
| 英文摘要: |
| Soil pollution with heavy metals is one of the most severe environmental and human health problems. Microbial remediation of heavy metal polluted soils has received increasing interests. Here a cadmium-resistant bacterial strain m6 that could activate cadmium was isolated from heavy metal-polluted soils in Fuyang City, Zhejiang Province, and was identified as Arthrobacter by 16S rDNA gene sequence analysis. Then, the effects of the isolated strain on heavy metal solubilization and soil microbial community structure were investigated in a pot experiment. Soil cadmium fractions were also evaluated using modified Tessier sequential extraction method. Inoculation of the isolate m6 increased the concentration of soluble cadmium by 43.47% and soil exchangeable cadmium by 16%, indicating the ability of the strain to solublize cadmium. After inoculation with the strain, the average well color development(AWCD) values of soil were increased by 14%~146% and the utilization of sugar and derivatives, amines, and polymer were also enhanced. Inoculation of cadmium-tolerant bacterial isolates increased total phospholipid fatty acid(PLFA) by 23.56%, and enhanced the fungal signature PLFAs(18:2ω6, 9、16:1ω5c、and 18:1ω9c) significantly, but reduced the actinomycete PLFAs(l0Me 16:0、l0Me 17:0 and 10Me 18:0) significantly, and decreased G-/G+ ratio by 38.6%. |
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