| 曹小闯,李晓艳,朱练峰,张均华,禹盛苗,金千瑜,吴良欢.外源甘氨酸态氮、硝态氮和铵态氮的浓度配比对小白菜生长和品质的影响[J].农业环境科学学报,2015,34(10):1846-1852. |
| 外源甘氨酸态氮、硝态氮和铵态氮的浓度配比对小白菜生长和品质的影响 |
| Effects of Different Ratios of Exogenous Glycine, Nitrate and Ammonium on Growth and Quality of Pakchoi(Brassica chinensis L.) |
| 投稿时间：2015-04-21 |
| DOI：10.11654/jaes.2015.10.002 |
| 中文关键词: 氮浓度与配比 氨基酸 生物量与品质 根系形态 局部无菌培养 |
| 英文关键词: nitrogen concentration and form ratio amino acid biomass and quality root morphology partial sterile cultivation |
| 基金项目:浙江省自然科学基金(LQ15C130004);国家自然科学基金(31172032,31172032,30900880);国家重点基础研究发展973计划(2015CB150502) |
| 作者 | 单位 | E-mail | | 曹小闯 | 中国水稻研究所, 水稻生物学国家重点实验室, 杭州 310006 | | | 李晓艳 | 浙江大学环境与资源学院, 教育部环境修复与生态健康重点实验室, 杭州 310058 | | | 朱练峰 | 中国水稻研究所, 水稻生物学国家重点实验室, 杭州 310006 | | | 张均华 | 中国水稻研究所, 水稻生物学国家重点实验室, 杭州 310006 | | | 禹盛苗 | 中国水稻研究所, 水稻生物学国家重点实验室, 杭州 310006 | | | 金千瑜 | 中国水稻研究所, 水稻生物学国家重点实验室, 杭州 310006 | | | 吴良欢 | 浙江大学环境与资源学院, 教育部环境修复与生态健康重点实验室, 杭州 310058 | finm@zju.edu.cn |
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| 中文摘要: |
| 为优化不同形态氮浓度配比,提高设施栽培蔬菜营养品质,在局部无菌水培条件下,研究了不同外源甘氨酸态氮、硝态氮和铵态氮的浓度配比对小白菜生物量、根系形态及营养品质的影响.结果表明,小白菜生物量及其营养品质受外源氮浓度组成影响差异显著(P <0.05).在高甘氨酸态氮(2500 μmol·L-1)和铵态氮(2500 μmol·L-1)、低硝态氮(250 μmol·L-1)浓度处理中,小白菜生物量最高,达94.5 mg·株-1,且小白菜总根长、根表面积、根体积、根系活力等指标的变化趋势与生物量一致;在高甘氨酸态氮、低无机氮浓度处理中,小白菜可溶性蛋白、游离氨基酸和可溶性糖含量最高,分别为26.2、126、24.0 mg·kg-1,且硝酸盐含量较高无机氮处理显著降低(P <0.05).适当提高甘氨酸态氮浓度、降低无机氮(尤其是硝态氮)浓度能够一定程度改善其营养品质. |
| 英文摘要: |
| Plants not only absorb nitrate(NO3--N) and ammonium(NH4+-N), but also have the ability to take up intact amino acids, such as glycine(Gly-N). It has been known that exogenous nitrogen(N) form in fertilizers is a major factor determining the nitrate accumulation and nutritional quality of plant edible parts. However, the effects of applying different N form mixture on plant growth and nutritional quality are still poorly understood. In this study, the effects of different concentrations and forms of exogenous Gly-N, NO3--N, and NH4+-N on biomass, root morphology and nutritional quality of pakchoi(Brassica chinensis L.) were explored under partial sterile hydroponic cultivation condition. Results showed that the biomass and nutritional quality of pakchoi was significantly affected by the ratios of three N forms(P <0.05). The highest pakchoi biomass(94.5 mg·plant-1) was achieved in the treatment supplied with high Gly-N(2500 μmol·L-1), high NH4+-N(2500 μmol·L-1), and low NO3--N(250 μmol·L-1). The total root length, total root surface area, total root volume, and root activity followed a similar variation trend to the pakchoi biomass. Simultaneous applications of high Gly-N and low inorganic N resulted in the highest content of total soluble proteins, total free amino acids, and total soluble sugars in pakchoi plants(about 26.2 mg·g-1, 126 mg·kg-1, and 24.0 mg·kg-1, respectively). Accumulation of nitrate was significantly decreased by high Gly-N and low inorganic N treatments, compared with the high inorganic N treatments(P <0.05). It suggests that appropriate formula of N sources, particularly higher Gly-N while in lower NO3--N, can partly improve the nutritional quality of pakchoi under sterile hydroponic conditions. Our results shed light on nutritional quality improvement of vegetables by optimizing the composition of different N forms in protected vegetable cultivation. |
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