| 薛培英,陈苗苗,耿丽平,赵全利,王亚琼.轮叶黑藻对无机砷的吸收和释放[J].农业环境科学学报,2015,34(10):1853-1857. |
| 轮叶黑藻对无机砷的吸收和释放 |
| Uptake and Release of Inorganic Arsenic by Hydrilla verticillata(L.f.) Royle |
| 投稿时间：2015-04-12 |
| DOI：10.11654/jaes.2015.10.003 |
| 中文关键词: 轮叶黑藻 砷 吸收 释放 |
| 英文关键词: Hydrilla verticillata arsenic uptake efflux |
| 基金项目:国家自然科学基金资助项目(21407042);河北省高等校院青年拔尖人才科学技术研究项目(BJ2014033) |
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| 中文摘要: |
| 采用室内模拟试验,通过对无机砷的吸收和释放研究,结合砷形态测定,研究了沉水植物轮叶黑藻[Hydrilla verticillata(L.f.) Royle]对无机砷(As)的吸收和释放能力.结果表明:黑藻对五价砷(AsⅤ)和三价砷(AsⅢ)均具有较高的吸收速率(最大吸收速率分别为105、96.1 nmol·g-1 DW·min-1),且对AsⅤ和AsⅢ的最大吸收速率差异不显著(P >0.05).无论暴露于AsⅤ还是AsⅢ溶液中,黑藻体内均以AsⅢ形态为主(> 90%),而且黑藻可以将外界AsⅤ迅速吸收进入体内并还原为AsⅢ,并快速将AsⅢ释放到外部溶液中(AsⅢ释放量占AsⅤ吸收量的53%).加磷(P)显著抑制黑藻对AsⅤ的富集(体内主要存在形态AsⅢ为不加P时的1/3),进一步导致溶液中的砷形态以AsⅤ为主(占总砷含量90%),加P不影响黑藻对AsⅢ的富集;植物螯合素(PCs)合成抑制剂丁胱亚磺酰亚胺(BSO)显著抑制黑藻对AsⅤ和AsⅢ的吸收,无论是AsⅤ还是AsⅢ处理,黑藻体内AsⅢ含量均降低,分别为对照处理的8%和10%,且溶液中砷形态含量与对照相比差异不显著(P >0.05). |
| 英文摘要: |
| Hydrilla verticillata(L.f.) Royle is a widespread submerged macrophyte in aquatic environments and shows high arsenic(As) acmulation. Hence, comprehensive understanding of uptake and release as well as species transformation of As in H. verticillata is essential for the development of phytoremediation technologies. Simulation experiments were conducted to investigate the uptake and efflux of AsⅤ and AsⅢ by H. verticillata cultivated in solution. Results showed that:the highest influx rates(Vmax) of AsⅤ and AsⅢ were 105 and 96.1 nmol·g-1 DW·min-1, respectively, with no significant difference in Vmax between AsⅤ and AsⅢ treatments(P >0.05). Regardless of As species supplied, AsⅢ was the predominant species in plant(> 90% of total extractable As). AsⅤ was rapidly taken up by H. verticillata from the growth medium, and then reduced to AsⅢ that released to the external solution(The ratios of AsⅢ efflux to AsⅤ uptake was 53%). Phosphorus addition significantly inhibited the uptake of AsⅤ by H. verticillata, with plant AsⅢ being 2/3 less than in-P treatment, but had little effects on AsⅢ uptake, resulting in the predominance of AsⅤ species in the external solution(over 90% of total As). BSO, a synthetic inhibitor of PCs, significantly reduced AsⅤ and AsⅢ accumulation in H. verticillata(AsⅢ concentration in the plant shoots was only 8% and 10% of that in the control, respectively). However, there were no differences in As species in solution between with and without BSO treatments. In conclusion, H. verticillata shows high capacity for As accumulation, but the rapid reduction of AsⅤ to AsⅢ followed by leakage of AsⅢ may limit the application of this plant in As phytoremediation. |
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