外源一氧化氮对镉胁迫下紫花苜蓿幼苗活性氧代谢和镉积累的影响

陈银萍; 蘧苗苗; 苏向楠; NGABIRE Maurice; 杨志娟; 杨述文; 摆玉林

文章摘要

陈银萍,蘧苗苗,苏向楠,NGABIRE Maurice,杨志娟,杨述文,摆玉林.外源一氧化氮对镉胁迫下紫花苜蓿幼苗活性氧代谢和镉积累的影响[J].农业环境科学学报,2015,34(12):2261-2271.

外源一氧化氮对镉胁迫下紫花苜蓿幼苗活性氧代谢和镉积累的影响

Effect of Exogenous Nitric Oxide on Active Oxygen Metabolism and Cadmium Accumulation in Alfalfa Seedlings Under Cadmium Stress

投稿时间：2015-06-24

DOI：10.11654/jaes.2015.12.003

中文关键词: 一氧化氮(NO) 紫花苜蓿膜脂过氧化抗氧化酶系统渗透调节物质 Cd积累

英文关键词: nitric oxide(NO) alfalfa(Medicogo sativa L.) membrane lipid peroxidation antioxidant enzyme osmotic-regulating substance cadmium accumulation

基金项目:国家自然科学基金项目(31260089,31560161,31170413)

作者	单位
陈银萍	兰州交通大学环境与市政工程学院, 兰州 730070
蘧苗苗	兰州交通大学环境与市政工程学院, 兰州 730070
苏向楠	兰州交通大学环境与市政工程学院, 兰州 730070
NGABIRE Maurice	兰州交通大学环境与市政工程学院, 兰州 730070
杨志娟	兰州交通大学环境与市政工程学院, 兰州 730070
杨述文	兰州交通大学环境与市政工程学院, 兰州 730070
摆玉林	兰州交通大学环境与市政工程学院, 兰州 730070

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中文摘要:

采用外源一氧化氮(NO)供体硝普钠(SNP)对镉(Cd)胁迫(0.5 mmol·L^-1 CdCl₂)下紫花苜蓿(Medicogo sativa L.)幼苗进行预处理,分析NO对Cd胁迫下紫花苜蓿幼苗膜脂过氧化、抗氧化酶系统、渗透调节物质和Cd积累量的影响,探讨NO在植物逆境胁迫响应中的作用及其机理。结果表明:300 μmol·L^-1 SNP显着降低Cd胁迫下紫花苜蓿幼苗相对电导率(REC)、丙二醛(MDA)含量、过氧化氢(H₂O₂)含量和超氧阴离子(O₂^-·)产生速率;400 μmol·L^-1 SNP显着促进脯氨酸(Pro)、可溶性蛋白(SP)、类胡萝卜素(Car)的合成;200 μmol·L^-1 SNP显着降低叶和茎中Cd含量;100 μmol·L^-1 SNP显着降低根中Cd含量。不同浓度SNP对紫花苜蓿叶、茎和根中抗氧化酶活性的影响比较复杂:100 μmol·L^-1 SNP显着增强叶中过氧化物酶(POD)活性、叶和茎中超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GR)活性;50 μmol·L^-1 SNP显着增强叶、茎和根中抗坏血酸酶(APX)活性;400 μmol·L^-1显着增强茎和根中POD活性。适当浓度的NO可以增加渗透调节物质含量和调节抗氧化酶活性,有效保护紫花苜蓿幼苗膜系统的稳定性,缓解Cd胁迫对紫花苜蓿幼苗的伤害。

英文摘要:

Nitric oxide(NO) is currently believed to play significant roles in plant responses to environmental changes. In this study, the effect of exogenous nitric oxide on the membrane lipid peroxidation, osmotic regulation substances, antioxidant enzyme activities and cadmium accumulation in leaves, stems, and roots of alfalfa seedlings and its mechanisms were studied under cadmium(0.5 mmol·L^-1 CdCl₂) stress using sodium nitropprusside(SNP) as NO donor. Supplying 300 μmol·L^-1 SNP remarkably reduced relative electric conductivity(REC), malondialdehyde(MDA) and hydrogen peroxide(H₂O₂) content, and superoxide anion(O₂^-·) -generating rate. Application of 400 μmol·L^-1 SNP significantly promoted the synthesis of proline(Pro), soluble protein(SP) and carotenoid(Car). The effect of SNP on activities of antioxidant enzymes in leaves, stems and roots of alfalfa seedlings was dependent on SNP concentrations. Treatment with 100 μmol·L^-1 SNP significantly enhanced activities of peroxidase(POD) in leaves and superoxide dismutase(SOD) and glutathione reductase(GR) in leaves and stems. Applying 50 μmol·L^-1 SNP significantly enhanced activities of ascorbic acid oxidase(APX) in leaves, stems and roots. The POD activity in stems and roots was significantly enhanced by 400 μmol·L^-1 SNP. Cadmium accumulation in leaves and stems was remarkably reduced by 200 μmol·L^-1 SNP, whereas root cadmium was obviously decreased by 100 μmol·L^-1 SNP. The results show that appropriate concentrations of exogenous nitric oxide could increase the content of osmotic-regulating substances and regulating activities of antioxidant enzymes to protect the membrane system stability and to alleviate Cd stress in alfalfa seedlings effectively.

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