| 赵雪松,任新,杨春维,尤宏,任百祥.BDE-47对斑马鱼胚胎氧化应激与DNA损伤的毒性研究[J].农业环境科学学报,2015,34(12):2280-2286. |
| BDE-47对斑马鱼胚胎氧化应激与DNA损伤的毒性研究 |
| Oxidative Stress and DNA Damage in Zebrafish Embryos Exposed to BDE-47 |
| 投稿时间：2015-06-24 |
| DOI：10.11654/jaes.2015.12.005 |
| 中文关键词: BDE-47 斑马鱼胚胎 氧化应激 DNA损伤 |
| 英文关键词: BDE-47 zebrafish embryos oxidative stress DNA damage |
| 基金项目:四平市科技发展计划项目(四科合字第2014050号);吉林师范大学2015年大学生创新创业计划项目 |
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| 中文摘要: |
| 以斑马鱼胚胎为受试对象,研究了2,2',4,4'-四溴联苯醚(BDE-47)对胚胎的氧化应激及DNA损伤。通过对不同浓度BDE-47(1、5、10、50 μg·L-1)暴露5 d后胚胎/幼鱼体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽S转移酶(GSH)的活性及丙二醛(MDA)、活性氧(ROS)含量变化的分析以及Sod1、Ucp-2、Gstp2及Nqo1基因相对量表达的测定,考查BDE-47对斑马鱼胚胎/幼鱼的氧化应激效应;通过单细胞凝胶电泳(SCGE)对尾矩值的分析,探讨BDE-47对斑马鱼胚胎/幼鱼的DNA损伤。结果显示,BDE-47能够显着诱导ROS与MDA的产生,并呈现浓度效应依赖关系;50 μg·L-1BDE-47能够显着增加SOD与CAT的活性,降低GSH的浓度。BDE-47暴露后50 μg·L-1暴露组基因Ucp-2的表达显着上调4.12倍(p<0.01),Gstp2与Nqo1表达分别显着下调至对照组的0.62倍与0.55倍(p<0.01),而基因Sod1相对表达量没有显着变化。同时,5、10、50 μg·L-1暴露组胚胎/幼鱼尾矩值显着增加并伴随着ROS的浓度升高。结果表明,BDE-47能够诱导斑马鱼胚胎/幼鱼产生氧化应激,ROS是诱发胚胎发生DNA损伤的导火索。 |
| 英文摘要: |
| Polybrominated diphenyl ethers(PBDEs), widely used flame retardants, have been detected in environment, biota, and even human blood serum. Currently, little is known about their toxicity to fish. This experiment was designed to explore the oxidative stress and DNA damage of 2,2',4,4'-tetrabromodi-phenyl ether(BDE-47) to zebrafish embryos. Superoxide dismutase(SOD), catalase(CAT), total glutathione(GSH), malondialdehyde(MDA), and relative mRNA expression levels of genes(Sod1、Ucp-2、Gstp2 and Nqo1) were assessed in zebrafish embryos exposed to 1, 5, 10, and 50 μg·L-1 of BDE-47 for 96 hours. The single cell gel electrophoresis was performed to observe the DNA damage caused by BDE-47. Compared with control, reactive oxygen species(ROS) and MDA were increased by BDE-47 treatments in a dose-effect manner. Exposure to 50 μg·L-1 of BDE-47 for 96 hours significantly increased the activities of SOD and CAT, but significantly decreased GSH levels. Moreover, the transcription of Ucp-2 was significantly up-regulated to 4.12 folds(p<0.01) while that of Gstp2 and Nqo1 was significantly down-regulated to 0.62 folds and 0.55 folds(p<0.01), respectively, by 50 μg·L-1 BDE-47. There was no significant difference observed in the expressions of Sod1 between the treatments and the control. Under exposure of 5, 10, and 50 μg·L-1, olive tail moment increased significantly with increasing concentrations of ROS. These results indicate that BDE-47 could induce oxidative stress in zebrafish embryos and ROS is the trigger inducing embryos DNA damage. |
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