| 张娟,魏克强,赵婷.Cu2+胁迫对克氏原螯虾(Procambarus clarkii)酚氧化酶原激活系统活性的影响[J].农业环境科学学报,2016,35(5):865-870. |
| Cu2+胁迫对克氏原螯虾(Procambarus clarkii)酚氧化酶原激活系统活性的影响 |
| Effect of Cu2+ stress on activity of prophenoloxidase activating system in Procambarus clarkii |
| 投稿时间：2015-11-08 |
| DOI：10.11654/jaes.2016.05.007 |
| 中文关键词: 克氏原螯虾 血蓝蛋白 酚氧化酶 丝氨酸蛋白酶 Cu2+ |
| 英文关键词: Procambarus clarkii hemocyanin phenoloxidase serine protease Cu2+ |
| 基金项目:教育部高等学校博士学科点专项科研基金(200801081012);山西省人才引进与开发专项基金项目(2006) |
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| 中文摘要: |
| 重金属铜是养殖水体的主要污染物,严重影响甲壳动物的免疫机能。以淡水克氏原螯虾(Procambarus clarkii)为实验对象,以亚致死浓度(1.0、3.0、5.0、10.0 mg·L-1,96-h LC50=22.14 mg·L-1)的Cu2+为胁迫因子,采用静态水质接触染毒法,通过分析血细胞内酚氧化酶原(proPO)、丝氨酸蛋白酶(SP)以及血淋巴中酚氧化酶(PO)、血蓝蛋白(Hc)和血细胞数(THC)的水平,探讨了Cu2+胁迫对酚氧化酶原激活系统(proPO-AS)活性的影响。结果表明,与未染毒的对照组相比,Cu2+显著抑制proPO、PO的活性以及THC、Hc的含量(P <0.05);但仅在10.0 mg·L-1 Cu2+浓度下,SP的活性显著降低(P <0.05)。研究结果提示,水体Cu2+对螯虾具有免疫毒性效应,氧化应激导致的血细胞数、血蓝蛋白含量、丝氨酸蛋白酶活性抑制可能是影响proPO-AS活性的主要机制之一。 |
| 英文摘要: |
| Copper(Cu) is one of ubiquitous contaminants in the aquatic environment, causing potential immunotoxicity to crustacean. In the present study, the effect of Cu2+ stress on prophenoloxidase activating system(proPO-AS), the most important component of innate defense, was evaluated in freshwater crayfish Procambarus clarkii. After 96 h exposure to sub-lethal concentrations(0, 1.0, 3.0, 5.0 mg·L-1 and 10.0 mg·L-1) of Cu2+, the activities of prophenoloxidase(proPO) and serine protease(SP) in hemocyte lysate supernatant(HLS) and the levels of total haemocyte count(THC), phenoloxidase(PO), and hemocyanin(Hc) in haemolymph were determined. Results indicated that there was a significant reduction(P<0.05) in crayfish immunocompetence, including proPO, PO, THC, and Hc, at higher Cu2+ concentrations. The SP activity was obviously inhibited only at 10.0 mg·L-1 Cu2+. The aqueous Cu2+ may play an important role in regulating proPO-AS by inhibiting THC, Hc, and SP. The immune factors, such as THC, proPO, and PO, could be considered as sensitive biomarkers of immunotoxicity in crayfish species. |
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