| 刘登璐,李廷轩,余海英,张路,王勇.不同烟草材料镉积累差异评价[J].农业环境科学学报,2016,35(11):2067-2076. |
| 不同烟草材料镉积累差异评价 |
| Evaluation of differential cadmium accumulation ability in different tobacco species |
| 投稿时间：2016-05-10 |
| DOI：10.11654/jaes.2016-0646 |
| 中文关键词: 烟草，镉，耐性，积累，差异评价 |
| 英文关键词: tobacco, cadmium, tolerance, accumulation, differential evaluation |
| 基金项目:四川省科技支撑计划项目(2014NZ0008)；四川省教育厅项目(14ZB0017) |
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| 摘要点击次数: 2200 |
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| 中文摘要: |
| 为进一步研究烟草镉积累遗传特性和培育烟草镉低积累品种提供材料，以93份烟草材料为研究对象，采用水培试验，以耐性指数和镉含量为指标对不同烟草材料镉积累进行评价，获取镉低积累种质资源，并分析其镉积累特征。结果表明：(1)两个镉浓度处理下，供试烟草材料地上部均受到不同程度的抑制，且材料间表现出显著差异，并以耐性指数为聚类分析指标，将材料分为高耐性、中耐性和低耐性3类，中高耐性材料生物量及耐性指数显著高于低耐性材料。(2)两个镉浓度处理下，中高耐性烟草材料镉积累能力存在较大差异，以镉含量为聚类分析指标，将中高耐性材料分为低积累、中积累和高积累3类，在2 mg·L-1镉处理下获得低积累材料12份，在10 mg·L-1镉处理下获得10份。(3)低积累、中积累和高积累材料地上部生物量和镉积累量均随着镉处理浓度的增加而下降，而其镉含量则表现出相反的变化趋势，烟草镉低积累材料地上部生物量与中高积累材料相比差异不显著，但其镉含量和镉积累量显著低于中高积累材料。该研究中，通过镉积累差异评价获得镉低积累种质资源7份，分别为“云烟85”、“中烟90”、“翠碧一号”、“CF986”、“RG11”、“91-58#”和“960116#”，这7份材料在两个镉处理下均表现出低积累特性，可为培育镉低积累烟草品种提供优良的遗传材料。 |
| 英文摘要: |
| A hydroponic experiment was conducted to obtain tobacco germplasm resources with lower Cd accumulation characteristics from 93 tobacco species by evaluating Cd tolerance index and accumulation, so as to provide species and information for the further researches on the genetic characteristics and cultivation of tobaccos with lower Cd accumulation characteristics, of which Cd accumulation characteristics were also analyzed. For the different treatments of Cd, the shoots of all tobaccos were inhibited and showed significantly difference among species. According to the cluster analysis, all species were classified as three types of high tolerance, middle tolerance and low tolerance. The biomass and the tolerance index of the high tolerance and middle tolerance species were significantly higher than those of the low tolerance species. There was observed a significant difference in Cd accumulation of middle tolerance and high tolerance species. According to the cluster analysis of Cd content, the medium tolerance and high tolerance species were classified as three types, which were low accumulation, middle accumulation and high accumulation species. 12 and 10 kinds of low accumulation species were obtained at the treatment of 2 mg·L-1 and 10 mg·L-1 Cd. The biomass and Cd accumulation of the low accumulation, middle accumulation and high accumulation species decreased with the increasing Cd concentrations, while Cd content in shoots of tobaccos increased with the increasing Cd concentrations. There was not noticed a significant difference in shoot biomass between the low accumulation species and other species, but Cd content and Cd accumulation of the low accumulation species were significant lower than other species. In this study, 7 kinds of tobacco species with lower Cd accumulation characteristics(Yuyan85", "Zhongyan90", "Cuibiyihao", "CF986", "91-58#" and "960116#) were obtained. And these provide excellent genetic materials for the cultivation of tobaccos with lower Cd accumulation characteristics. |
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