| 赵书晗,王海娟,王宏镔.砷胁迫下吲哚乙酸对不同砷富集能力植物光合作用的影响[J].农业环境科学学报,2017,36(6):1093-1101. |
| 砷胁迫下吲哚乙酸对不同砷富集能力植物光合作用的影响 |
| Effect of indoleacetic acid on photosynthesis of arsenic-stressed plants with different arsenic-accumulating ability |
| 投稿时间：2017-01-17 |
| DOI：10.11654/jaes.2017-0092 |
| 中文关键词: 吲哚乙酸 砷 超富集植物 光合作用 超微结构 |
| 英文关键词: indoleacetic acid arsenic hyperaccumulator photosynthesis ultrastructure |
| 基金项目:国家自然科学基金项目（31360132）；云南省中青年学术和技术带头人后备人才项目（2012HB007） |
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| 中文摘要: |
| 以砷超富集植物大叶井口边草（Pteris cretica var. nervosa）和非超富集植物剑叶凤尾蕨（Pteris ensiformis）为供试植物，研究2 mg·L-1 As（Ⅴ）胁迫下添加不同浓度吲哚乙酸（IAA）对2种植物株高、生物量、叶片砷含量、光合色素、叶绿素荧光参数、暗反应酶活性和叶绿体超微结构的影响。结果表明：添加20 mg·L-1 IAA后，2种植物生物量和叶片砷含量与对照相比显着增加，且大叶井口边草叶片砷含量显着高于剑叶凤尾蕨；大叶井口边草叶片光合色素含量与对照相比均无显着差异，而剑叶凤尾蕨则显着降低。随着IAA浓度增加，大叶井口边草叶片叶绿素a/b值与对照相比无显着差异，而剑叶凤尾蕨在40 mg·L-1 IAA处理时显着降低。大叶井口边草叶片最大光化学效率（Fv/Fm）、实际光化学效率（ΦPSII）和非光化学淬灭系数（qN）随IAA浓度增加与对照相比无显着差异，而剑叶凤尾蕨在20 mg·L-1 IAA处理下开始显着下降；大叶井口边草叶片光化学淬灭系数（qP）在10、40 mg·L-1 IAA处理时显着增加，而在剑叶凤尾蕨中则显着下降。随IAA浓度增加，剑叶凤尾蕨叶片核酮糖-1，5-二磷酸羧化酶活性与对照相比显着下降，而大叶井口边草仅在10 mg·L-1 IAA处理下显着下降，在20 mg·L-1 IAA处理下则显着增加，在40 mg·L-1 IAA处理下与对照无显着差异。添加IAA后，剑叶凤尾蕨叶片叶绿体超微结构受害严重，而大叶井口边草在20 mg·L-1 IAA处理时，叶绿体仍保持完好。因此，20 mg·L-1 IAA处理对砷胁迫下大叶井口边草的生长发育具有一定的光合保护作用，可使其既保持正常生长又超量富集砷。 |
| 英文摘要: |
| A hydroponic experiment was conducted to investigate the stress effect of arsenic(As) on Pteris cretica var. nervosa and Pteris ensiformis in the presence of indoleacetic acid(IAA). The growth, arsenic(As) accumulation, photosynthetic pigment content, chlorophyll fluorescence parameters, activities of two dark-reaction enzymes, and chloroplast ultrastructure of the two plants were evaluated to identify the stress effect at different IAA concentrations. The results showed that biomass and frond As concentration of the two plants were significantly increased in the presence of 20 mg·L-1 IAA compared to those in the respective control. The concentration of As in P. cretica var. nervosa fronds was much higher than that in P. ensiformis fronds. The photosynthetic pigment content in P. cretica var. nervosa fronds was similar to that of the control samples, but was significantly decreased in P. ensiformis. Increasing IAA concentrations did not affect the chlorophyll a/b value of P. cretica var. nervosa, compared to the control samples. However, the chlorophyll a/b value of P. ensiformis was significantly reduced in the presence of 40 mg·L-1 IAA. The maximal photochemical efficiency of photosystem Ⅱ(Fv/Fm), actual quantum yield(ΦPSII), and non-photochemical quenching coefficient(qN) remained unchanged in P. cretica var. nervosa fronds with increasing IAA concentrations, but decreased profoundly in P. ensiformis, especially in the presence of 20 mg·L-1 IAA. The photochemical quenching coefficient(qP) increased significantly in P. cretica var. nervosa but diminished significantly in P. ensiformis at 10 or 40 mg·L-1 IAA. The activity of ribulose-1,5-bisphosphate carboxylase(RuBPC) reduced significantly in P. ensiformis fronds with increasing IAA concentration. The RuBPC activity of P. cretica var. nervosa increased significantly at 20 mg·L-1 IAA but decreased at 10 mg·L-1 IAA, compared to the control. IAA addition severely damaged the ultrastructure of chloroplasts in P. ensiformis at all levels of IAA treatment but no visible damage was detected in P. cretica var. nervosa at 20 mg·L-1 IAA treatment. Therefore, 20 mg·L-1 IAA could protect the phytosynthesis of P. cretica var. nervosa under As stress, resulting in normal growth and hyperaccumulation of As. |
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