| 庞海东,贺卓,燕传明,盛下放,何琳燕.耐重金属的植物促生芽孢杆菌筛选及其强化香蒲去除Cd的作用[J].农业环境科学学报,2017,36(11):2314-2321. |
| 耐重金属的植物促生芽孢杆菌筛选及其强化香蒲去除Cd的作用 |
| Isolation of heavy metal resistant and plant growth promoting Bacillus strains and effects on cadmium removal by Typha angustifolia |
| 投稿时间：2017-05-04 |
| DOI：10.11654/jaes.2017-0648 |
| 中文关键词: 芽孢杆菌 狭叶香蒲 水体重金属 污染修复 Cd |
| 英文关键词: Bacillus species Typha angustifolia heavy metal contaminated waters remediation cadmium |
| 基金项目:国家自然科学基金项目（41471273） |
| 作者 | 单位 | E-mail | | 庞海东 | 农业部农业环境微生物重点实验室, 南京农业大学生命科学学院, 南京 210095 | | | 贺卓 | 农业部农业环境微生物重点实验室, 南京农业大学生命科学学院, 南京 210095 | | | 燕传明 | 农业部农业环境微生物重点实验室, 南京农业大学生命科学学院, 南京 210095 | | | 盛下放 | 农业部农业环境微生物重点实验室, 南京农业大学生命科学学院, 南京 210095 | | | 何琳燕 | 农业部农业环境微生物重点实验室, 南京农业大学生命科学学院, 南京 210095 | helyan0794@njau.edu.cn |
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| 中文摘要: |
| 为探讨细菌对水生植物修复水体重金属污染的影响，从南京某矿区废弃地的湿地植物柳树（Salix babylonica）、芦苇（Phragmites australis）、香蒲（Typha latifolia）根际和根内分离筛选到8株芽孢杆菌，这些芽孢杆菌都具有产铁载体、吲哚乙酸、多糖和淀粉酶活性，且对重金属的抗性较强，对温度、pH和盐浓度具有一定的耐受性。采用水培试验研究了Cd胁迫下耐重金属植物促生芽孢杆菌强化狭叶香蒲（T.angustifolia）吸收积累Cd的作用，结果表明，在2.5 mg·L-1 Cd2+的条件下接种芽孢杆菌处理能够促进香蒲生长和组织中Cd含量增加。其中Bacillus megaterium P24和P37促进香蒲根和地上部干重显著增加40.0%~46.4%，B.amyloliquefaciens P29能使地上部Cd含量和总吸收量显著增加31.1%和63.5%；溶液中Cd去除率最高可达79.5%。研究表明，耐重金属芽孢杆菌能够促进香蒲生长、增加香蒲Cd含量，从而增加香蒲Cd总吸收量，达到去除水中Cd污染的作用，具有应用于水体重金属污染修复的潜力。 |
| 英文摘要: |
| Metal resistant bacteria provide an effective way to enhance heavy metal removal rates of wetland phytoremediation systems. In this work, heavy metal resistant rhizobacteria and endophytic bacteria were isolated from plants growing in the waste mine wetland in Nanjing, Jiangsu, China. The effects of the bacteria on plant growth and cadmium removal by the narrow-leaved cattail(Typha angustifolia L.) under excessive cadmium exposure were investigated. The results indicated that the eight metal resistant Bacillus strains isolated from the aquatic plants showed a variety of growth promoting features including solubilizing phosphate and producing indole acetic acid(IAA), siderophores, polysaccharides, and amylase. The isolates showed resistance to Cd, Pb, Cu, and Zn and tolerance to acids, alkalis, and salts. The growth of T.angustifolia was greatly promoted and the content of Cd in T.angustifolia increased after inoculation of the Bacillus strains into a Cd2+-containing solution. The dry weights of the root and aboveground tissues of T.angustifolia inoculated with Bacillus megaterium P24 and P37 were significantly increased by 40.0%~46.4%, compared with the control. Besides, the Cd content(31.1%) and total Cd uptake(63.5%) in the aboveground tissue increased when inoculated with Bacillus amyloliquefaciens P29. The highest Cd removal rate was 79.5%, after inoculation with B. megaterium P24. The results suggest that the metal resistant B.amyloliquefaciens P29 and B. megaterium P24 and P37 could be used as effective inoculants for improved phytoremediation in metal polluted waters. |
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