| 王晓旭,孙丽娜,郑学昊,吴昊,王辉,刘春跃.DDTs污染农田土壤的强化微生物修复研究[J].农业环境科学学报,2018,37(1):72-78. |
| DDTs污染农田土壤的强化微生物修复研究 |
| Enhanced microbial remediation of dichlorodiphenyltrichloroethane(DDT)-contaminated farmland soil |
| 投稿时间：2017-06-20 |
| DOI：10.11654/jaes.2017-0882 |
| 中文关键词: DDTs 混合菌 阴-非离子混合表面活性剂 血粉 |
| 英文关键词: DDTs mixed bacteria anionic-nonionic mixed surfactant blood meal |
| 基金项目:国家重点基础研究发展计划项目（973计划）（2014CB441106）；中华环境保护基金会格平绿色行动辽宁环境科研教育"123"工程项目（CEPF2013-123-1-7）；沈阳市科学事业费竞争性选择项目（城市生态环境风险管理及其修复技术研究）；辽宁省自然科学基金指导计划项目（20170540656）；我国1：5万土壤图籍编撰及高精度数字土壤构建（二期工程）项目（2012FY112100） |
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| 中文摘要: |
| 为了提高微生物对DDTs污染土壤的修复效率，利用混合菌[球形节杆菌（Arthrobacter globiformis）和甲基营养型芽孢杆菌（Bacillus methylotrophicus）]对沈阳市沈北新区设施农业DDTs污染土壤进行现场修复实验，研究定期添加血粉（3X）和阴-非离子混合表面活性剂SDBS-Tween 80（3H）对混合菌修复DDTs污染土壤的强化效果，分析修复过程对DDT同分异构体及初级代谢产物的影响。结果表明：接种混合菌可有效降低土壤中DDTs的残留，连续3次接种的修复效果（3PN-1）比1次（PN-1）好，90 d后DDTs降解率可达50.5%，比对照组提高了45.9%。在此基础上，添加血粉和SDBS-Tween 80均可显著降低土壤中DDTs的残留，其中SDBS-Tween 80的处理效果较好，DDTs降解率最高达到63.2%，比单独接种混合菌处理提高了12.8%。DDT同分异构体及初级代谢产物分析表明，SDBS-Tween 80强化修复对生态毒性较强的p，p'-DDE降解率达到了62.4%，有效降低了修复后的生态风险。因此，SDBS-Tween 80强化混合菌处理可以作为DDTs污染土壤微生物修复的适宜措施。 |
| 英文摘要: |
| In order to improve the microbial remediation efficiency of dichlorodiphenyltrichloroethane(DDT)-contaminated soil, field-scale bioremediation of farmland soil contaminated by DDTs from the Shenyang North New Area of China was performed using mixed bacteria(Arthrobacter globiformis and Bacillus methylotrophicus). Enhanced mixed-bacteria remediation was investigated in the presence of blood meal(3X) and SDBS-Tween 80-mixed surfactants(3H). The changes in DDT isomers/primary metabolites during the remediation process were analyzed. The results showed that the inoculation of mixed bacteria could effectively reduce the residual DDTs in soil and the effect of three fold inoculation(3PN-1) was better than single inoculation(PN-1). After 90 days, the degradation rate of DDTs reached 50.5%, which was 45.9% higher than that of the control group. The residual DDTs in soil could be significantly reduced by adding blood meal and surfactants. Among the treatments, that of SDBS-Tween 80 combined with mixed bacteria was the best. The highest degradation rate of DDTs was 63.2%, which was 12.8% higher than that of the treatment of using mixed bacteria alone. The analysis result of the DDT isomers/primary metabolites indicated that remediation with SDBS-Tween 80 combined with mixed bacteria has a high degradation of 62.4% for p,p'-DDE, which has strong ecotoxicity, thus the ecological risk after remediation was effectively reduced. Therefore, SDBS-Tween 80-enhanced mixed-bacteria treatment has potential application for microbial remediation of DDT-contaminated soil. |
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