| 李江遐,张军,马友华,蔡慢弟,高飞.硅对镉胁迫条件下两个水稻品种镉亚细胞分布、非蛋白巯基物质含量的影响[J].农业环境科学学报,2018,37(6):1066-1071. |
| 硅对镉胁迫条件下两个水稻品种镉亚细胞分布、非蛋白巯基物质含量的影响 |
| Effects of silicon on cadmium accumulation and non-protein thiol content in the seedlings of two rice varieties under cadmium stress |
| 投稿时间：2017-07-03 |
| DOI：10.11654/jaes.2017-0928 |
| 中文关键词: 水稻幼苗 镉 硅 非蛋白巯基 亚细胞分布 |
| 英文关键词: rice seedling cadmium silicon non-protein thiol sub-cellular distribution |
| 基金项目:安徽省教育厅重点项目（KJ2014A072）；农业部农业生态环境保护项目（农财发〔2013〕16号） |
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| 中文摘要: |
| 采用营养液培养方法，以两种籽粒镉含量不同的水稻品种宁粳4号和徽两优6号为试验材料，分别生长在含有硅（Na2SiO3）和50 μmol·L-1镉（CdCl2）的介质中7 d，研究了硅对两种水稻吸收累积镉及其非蛋白巯基物质（NPT）含量的影响，比较了两种水稻地上部和根中镉的亚细胞分布特征。研究结果表明：在不同硅镉处理下，相同镉处理浓度下，加入硅后，两种水稻生物量较单独镉处理明显增加；两种水稻根中的镉含量明显高于地上部，籽粒镉含量低的水稻品种宁粳4号转移系数小于籽粒含量高的徽两优6号；外源硅（1.8 mmol·L-1）会显著降低水稻对镉的吸收和转运。镉胁迫条件下，硅对水稻植株体内的非蛋白巯基物质（NPT）的含量产生了影响，单独镉处理时两种水稻根部NPT含量较对照明显升高，硅镉复合处理时宁粳4号根NPT含量变化不明显但显著降低了地上部NPT含量，徽两优6号根中NPT含量增加，地上部NPT含量下降。两种水稻品种镉在根中的亚细胞分布表现为细胞可溶物质中含量高于细胞壁，细胞器上含量最少；镉在地上部的亚细胞分布表现为细胞壁中含量高于细胞可溶物质，细胞器上含量最少。 |
| 英文摘要: |
| Many studies have revealed that silicon(Si) enhances plant growth and decreases cadmium(Cd) accumulation in the shoots on rice. In this study, we examined Cd content and its subcellular distribution in two rice varieties(NJ4 and HLY6) using hydroponic experiments with treatments of Si(Na2SiO3) and Cd(50 μmol·L-1, CdCl2) for 7 days. The effects of Si on the contents of Cd and non-protein thiols(NPT) in both shoots and roots were examined. The results showed that under the different Si and Cd treatments, significant differences were found in the biomass of the two rice varieties. Both Si treatments significantly increased rice biomass. The Cd content in roots was higher than that in the aboveground parts. Transfer factors of the rice variety with the low grain Cd content(NJ4) were lower than those in the rice variety with high grain Cd content(HLY6). Si significantly reduced Cd accumulation and translocation in rice in the 50 μmol·L-1 Cd treatment. Under Cd stress, the content of NPT in rice was affected by Si. With Cd treatment alone, the content of NPT in roots of the two varieties was significantly higher than that of the control. Combined Cd-Si treatment resulted in no significant changes in the content of NPT in the roots of rice NJ4, but significantly decreased the content of NPT in stems. Cd-Si treatment also significantly increased the content of NPT in the roots of HLY6 but decreased the content of NPT in the stems of HLY6. In addition, the subcellular distributions of Cd in rice roots indicated that the content of Cd in the cytosol was higher than that in cell walls, and was lowest in cell organelles. Rice shoots had a higher content of Cd in cell walls than in the cell cytosol, with the lowest content being observed in cell organelles. |
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