| 吴东墨,王宏镔,王海娟,王忠振,蔡文昌.吲哚乙酸和激动素配合施用对蜈蚣草土壤砷提取效率的影响[J].农业环境科学学报,2018,37(8):1705-1715. |
| 吲哚乙酸和激动素配合施用对蜈蚣草土壤砷提取效率的影响 |
| Effects of the combined application of indole acetic acid and kinetin on the arsenic extraction efficiency of soil after planting Pteris vittata |
| 投稿时间：2018-01-22 |
| DOI：10.11654/jaes.2018-0122 |
| 中文关键词: 吲哚乙酸 激动素 蜈蚣草 砷(As) 生理生化 |
| 英文关键词: indole acetic acid kinetin Pteris vittata arsenic physiological and biochemical properties |
| 基金项目:国家自然科学基金项目（31360132）；云南省重点研发计划项目——云南省污染土壤修复关键技术研究与应用示范 |
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| 中文摘要: |
| 为研究植物激素吲哚乙酸（IAA）和激动素（KT）配合施用对砷（As）超富集植物蜈蚣草（Pteris vittata）As提取效率的影响，采用2因素4水平正交盆栽实验，在含As 55 mg·kg-1的土壤中，通过在叶面配合施用不同浓度IAA（0、25、50、100 mg·L-1）和KT（0、20、40、60 mg·L-1）筛选出使蜈蚣草As提取效率最高的配比，并分析最佳配比下导致蜈蚣草As提取效率最高的生理生化机制。结果表明：在16种IAA和KT配比浓度中，25 mg·L-1 IAA和20 mg·L-1 KT配合施用时蜈蚣草的As提取效率最高，达到6.49%。该最佳配比下，与未施用激素或单一激素的处理相比，蜈蚣草株高和干重均显著增加；根系形态得到有效改善，根系活力、光合色素含量及叶片过氧化物酶（POD）活性均显著增加，细胞膜脂过氧化产物丙二醛（MDA）含量显著降低。相关分析表明，在25 mg·L-1 IAA和20mg·L-1 KT配合施用下，蜈蚣草As提取效率最高的原因是激素明显改善了其地上部干重、根长、根系活力、光合色素含量及叶片POD活性，这些指标与其As提取效率呈显著正相关。 |
| 英文摘要: |
| The objective of this study is to examine the effects of the combined use of indole acetic acid(IAA)and kinetin(KT)on the arsenic(As)extraction efficiency of the As hyperaccumulator Pteris vittata. The orthogonal pot experiments were designed with two factors and four levels, using soil containing 55 mg·kg-1 of As. IAA(0, 25, 50, 100 mg·L-1)and KT(0, 20, 40, 60 mg·L-1)were applied to leaves, and the optimized dosages of the two plant hormones to achieve the highest As extraction efficiency in P. vittata were identified. Meanwhile, the related physiological and biochemical mechanisms were also explored. The results showed that among 16 different combinations of IAA and KT, the highest As extraction efficiency in P. vittata(up to 6.49%)was observed following the combined application of 25 mg·L-1 IAA and 20 mg·L-1 KT. Under this optimized condition, the height and dry weight of P. vittata were significantly higher than those in other treatments, including those where the plant hormones were absent or applied individually. After the two plant hormones were applied, the plant root morphology was effectively improved, and the root activity, content of photosynthetic pigments, and frond peroxidase(POD)activity were also significantly increased. The content of malondialdehyde(MDA), a peroxidation product of membrane lipids, was significantly reduced. Correlation analysis demonstrated the best As extraction efficiency of P. vittata due to the combined application of 25 mg·L-1 IAA and 20 mg·L-1 KT could be attributed to the fact that the foliar-applied plant hormones significantly improved the above-ground dry weight, root length, root activity, photosynthetic pigment content, and frond POD activity in P. vittata. |
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