文章摘要
石峰,魏晓雪,冯剑丰,朱琳.不同无机氮条件下一种硅藻的氮吸收动力学及模型预测分析[J].农业环境科学学报,2018,37(9):1833-1841.
不同无机氮条件下一种硅藻的氮吸收动力学及模型预测分析
Nitrogen uptake kinetics of a diatom and model prediction analysis in different inorganic nitrogen conditions
投稿时间:2018-01-27  
DOI:10.11654/jaes.2018-0154
中文关键词: 铵态氮  硝态氮  氮吸收动力学  铵氮抑制效应  模型
英文关键词: ammonium  nitrate  nitrogen uptake kinetics  ammonium inhibition  model
基金项目:国家自然科学基金项目(31470536);天津市自然科学基金项目(15JCYBJC22800)
作者单位E-mail
石峰 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300071  
魏晓雪 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300071  
冯剑丰 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300071  
朱琳 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300071 zhulin@nankai.edu.cn 
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中文摘要:
      针对海水体系中藻类对无机氮源的吸收利用以及氮源之间相互关系,在室内条件下,利用单细胞藻一次性培养的方法,以无机氮源铵态氮(NH4+-N)和硝态氮(NO3--N)为基础,通过改变氮源的形态及比例,研究了三角褐指藻(Phaeodactylum tricornutum)的生长动力学和氮吸收动力学特征,并通过构建动力学模型对该特征进行拟合。结果表明,同浓度不同形态的无机氮源培养中,三角褐指藻生长无显著差异(P>0.05)。分别以NH4+-N和NO3--N作为唯一氮源时,三角褐指藻能够快速对氮源吸收利用,而两种氮源同时存在时,藻类优先利用NH4+-N,NO3--N的吸收则受到一定的抑制。同时,所构建的模型较好地预测了藻类的生长动力学和氮吸收动力学趋势。
英文摘要:
      To investigate the utilization of inorganic nitrogen in the water by marine phytoplankton and the relationship between nitrogen sources, the growth curve and the nitrogen (ammonium and nitrate) uptake characteristics of Phaeodactylum tricornutum under different nitrogen culture conditions were measured in the laboratory. Moreover, a kinetic model was developed to predict these nitrogen uptake processes. The results showed that there was no significant difference in the growth of P. tricornutum (P>0.05) when reared in the same concentration but different forms of inorganic nitrogen cultures. Moreover, either of the two nitrogen forms could be taken up rapidly by Phaeodactylum tricornutum when they were present as the sole nitrogen form, but in mixed nitrogen cultures, nitrate uptake was clearly inhibited in the presence of ammonium. Additionally, the refined kinetic model can accurately predict the nitrogen uptake process in the tests.
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