| 刘树丽,段正洋,徐龙乾,徐晓军,宋淑敏,张梦娇.克雷伯氏菌活细胞和死细胞对水中Pb(Ⅱ)的去除性能[J].农业环境科学学报,2018,37(10):2278-2288. |
| 克雷伯氏菌活细胞和死细胞对水中Pb(Ⅱ)的去除性能 |
| Performance of live and dead cells of Klebsiella sp. in removing Pb (Ⅱ)from aqueous solution |
| 投稿时间：2018-01-09 |
| DOI：10.11654/jaes.2018-0050 |
| 中文关键词: 克雷伯氏菌 活细胞 死细胞 吸附 Pb(Ⅱ) |
| 英文关键词: Klebsiella sp. live cells dead cells adsorption Pb (Ⅱ) |
| 基金项目:云南省科技计划项目（2013FZ031）；昆明理工大学分析测试基金项目（2016P2014607005） |
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| 中文摘要: |
| 为了探究克雷伯氏菌的活细胞（LC）和经过高温处理后的死细胞（DC）作为吸附剂对水中Pb（Ⅱ）的去除性能以及两种吸附剂吸附能力的差异，系统研究了溶液pH值、吸附剂用量、反应时间和Pb（Ⅱ）初始浓度对两种吸附剂吸附能力的影响。利用扫描电镜（SEM）、比表面积测定、Zeta电位分析、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）等技术表征分析了LC和DC对Pb（Ⅱ）的吸附差异。结果显示，DC和LC对Pb（Ⅱ）的吸附动力学和吸附等温线分别能够较好地使用准二级动力学模型和Langmuir模型拟合。在温度为30℃的条件下，Langmuir模型预测DC和LC对Pb（Ⅱ）的最大吸附量分别为134.92 mg·g-1和116.18 mg·g-1。热力学参数研究表明DC对Pb（Ⅱ）的吸附为自发的放热过程，然而LC对Pb（Ⅱ）的吸附为自发的吸热过程。研究表明：DC比LC具有更大的比表面积和带有更多的负电荷，可能是由于高温处理破坏了细胞壁的完整性，使细胞壁的渗透性增强且DC表面暴露出更多氨基基团，从而使得在各条件下DC对Pb（Ⅱ）的吸附量明显高于LC。 |
| 英文摘要: |
| Live cells (LC) and dead cells (DC) of Klebsiella sp. were used as adsorbents to remove Pb (Ⅱ) from aqueous solutions. The dead cells were generated by sterilizing the cell cultures. The ability of LC and DC to remove Pb (Ⅱ) was compared and differences in adsorption capacity of above adsorbents were investigated in this work, through systematically studying factors influencing the adsorption and removal, including solution pH, adsorbent dosage, contact time, and initial Pb (Ⅱ) concentration. In addition, scanning electron microscopy (SEM), specific surface area determination, zeta potential analysis, fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) were performed to further illustrate the difference in adsorption performances. The results revealed that Pb (Ⅱ) adsorption by DC and LC fits well with pseudo-second-order kinetics and the Langmuir isotherm model according to adsorption kinetic and isotherm analyses, respectively. The maximum adsorption capacity of Pb (Ⅱ) was 134.92 mg·g-1 for DC and 116.18 mg·g-1 for LC at 30℃, determined by the Langmuir isotherm model. The thermodynamic parameters, such as △G, △H, and △S, showed that the adsorption of Pb (Ⅱ) by DC was spontaneous and exothermic, whereas it was spontaneous and endothermic for LC. Our results indicated that DC had a larger specific surface area and more negative charges than LC, possibly because the DC cell wall structure was no longer intact following high temperature treatment, increasing the permeability of the cell wall, thereby exposing more amino groups to the surface. The above difference resulted in DC presenting greater adsorption capacity for Pb (Ⅱ) than LC under the described experimental conditions. |
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