| 李爽,左尚武,王万清,王金嵩,权成伟,朱雪竹.菌株Serratia sp.PW7不同定殖方式对黑麦草中芘污染去除及其内生菌群的影响[J].农业环境科学学报,2018,37(12):2755-2764. |
| 菌株Serratia sp.PW7不同定殖方式对黑麦草中芘污染去除及其内生菌群的影响 |
| Determining the effects of Serratia sp. PW7 on pyrene removal and the endophytic bacterial community in ryegrass (Lolium multiflorum L.)via different inoculation methods |
| 投稿时间：2017-12-26 |
| DOI：10.11654/jaes.2017-1756 |
| 中文关键词: 芘 功能内生细菌 定殖 内生细菌种群 |
| 英文关键词: pyrene functional endophytic bacteria inoculation endophytic bacterial community |
| 基金项目:国家自然科学基金项目（31670514）；上海市"科技创新行动计划"长三角科技联合攻关领域项目（17295810600）；南京农业大学SRT（1613C28） |
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| 中文摘要: |
| 为探究功能植物内生细菌对植物体内多环芳烃（PAHs）污染的去除以及机理，选择芘为多环芳烃代表、黑麦草为修复植物，采用水培体系检测内生细菌Serratia sp.PW7定殖对黑麦草体内可培养内生细菌群落和芘污染去除的影响。结果表明：菌株PW7能够高效定殖在黑麦草根（5.87~7.63 lg CFU·g-1）和茎叶（3.49~4.97 lg CFU·g-1）中，促进植物生长和芘的去除，改变黑麦草体内内生菌群结构，提高黑麦草体内可培养细菌总数和多样性。内生细菌定殖对植株生长与芘去除的促进作用与菌株定殖效率正相关。比较浸根和浸种两种定殖方式，浸根具有较高的定殖效率与降芘效益，还可促进植物生长。浸根定殖后，黑麦草生长量提高了18.5%~28.0%，植株体内芘浓度降低35.7%~44.2%；同时，浸根处理的黑麦草在高浓度芘污染下，根中内生细菌多样性及均匀度达到试验中的最高值（H=2.22，J=0.865）。芘污染条件下，功能菌定殖可改变植株体内优势种，定殖后黑麦草根中Serratia属成为绝对优势属，Pantoea属、Erwinia属（高浓度）和Micrococcus属次之；茎叶中优势属Microbacterium属不变，另一优势属由Chryseobacterium属变为Pantoea属（低浓度）、Pseudomona属和Sphingobacterium属。体外芘降解实验证明，有7株优势内生细菌15 d芘降解率超过55%。实验结果表明，功能菌株PW7可通过定殖提高植株体内内生细菌多样性及改变其优势种群来降低植物体内芘污染。 |
| 英文摘要: |
| To understand the mechanism by which PAHs (polycyclic aromatic hydrocarbons) are removed from plants by functional endophytic bacteria, pyrene residues and cultivable endophytic bacterial communities in a ryegrass plant inoculated with Serratia sp. strain PW7 were investigated. The results showed that the PW7 strain of Serratia sp. could efficiently colonize in the roots (5.87~7.63 lg CFU·g-1) and shoots (3.49~4.97 lg CFU·g-1) of the inoculated seedlings. Colonization by PW7 strain significantly increased pyrene removal, plant growth, and the cell counts and diversities of cultivable endophytic bacteria. Remarkably, pyrene removal efficiency and plant growth were both positively correlated with the establishment efficiency of PW7 strain. Compared to the seedlings inoculated by soaking the seeds, more cells of the PW7 strain successfully colonized in the seedlings inoculated by dipping the roots, leading to less pyrene residues. Inoculated by dipping the roots enhanced the growth of inoculated seedlings by 18.5%~28.0% and decreased the pyrene concentation by 35.7%~44.2%. Meanwhile, the maximal indices of diversity and evenness (H=2.22, J=0.865) were found in the roots of the seedlings from group LR grown in Hoagland solution with 0.5 mg·L-1 pyrene. The endophytic bacterial population could be shifted by PW7 strain as the seedlings were exposed to pyrene. For the seedlings grown in the Hoagland solution with pyrene, the most dominant bacteria was Serratia, and the other dominant bacteria were Pantoea, Erwinia, and Micrococcus in the roots of inoculated seedlings. Whereas, in the shoots of inoculated seedlings grown in the Hoagland solution with pyrene, the most dominant bacteria remained Microbacterium, and other dominant bacteria shifted from Chryseobacterium to Pantoea, Pseudomonas, and Sphingobacterium. Moreover, seven strains of these dominant bacteria could degrade more than 55% of the pyrene in the media within 15 d. Our results clarified that functional endophytic PW7 strain could effectively increase the pyrene removal efficiency by increasing endophytic bacterial diversities and shifting the dominant endophytic bacteria. |
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