| 黄兰林,梅馨月,李详,李想,祖艳群,何永美,李元.UV-B辐射对稻瘟病菌侵染阶段四个致病相关基因表达的影响[J].农业环境科学学报,2019,38(3):494-501. |
| UV-B辐射对稻瘟病菌侵染阶段四个致病相关基因表达的影响 |
| Effects of UV-B radiation on the expression of four pathogenic genes in the infection stage of Magnaporthe grisea |
| 投稿时间：2018-05-12 |
| DOI：10.11654/jaes.2018-0625 |
| 中文关键词: UV-B 荧光定量PCR 致病性 稻瘟病菌 侵染过程 |
| 英文关键词: UV-B real-time PCR pathogenicity rice blast infection process |
| 基金项目:国家自然科学基金项目（31460141，31760113，41565010）；云南省农田无公害生产创新团队（2017HC015） |
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| 中文摘要: |
| 通过对稻瘟病菌侵染阶段4个致病基因在UV-B辐射处理下表达的研究，探索UV-B辐射影响稻瘟病菌致病力的机理。试验利用紫外灯模拟0、2.5 kJ·m-2和5 kJ·m-2的辐射强度处理稻瘟病菌，采用实时荧光定量PCR，对4个致病相关基因Chitinase、MGP1、MAGB、CPKA的表达进行分析。结果表明随着UV-B辐射剂量的增加，MGP1的表达没有显著变化；而Chitinase、MAGB、CPKA表达下调，在去除UV-B后6、24 h，其表达都有所回升。其中Chitinase和CPKA的表达整体受5 kJ·m-2强度影响较大，尤其在辐射120 min时表达分别下调了94%和61%。MAGB的表达变化则在2.5 kJ·m-2时更大，从辐射开始到结束，表达分别下调了57%、17%和40%。从对应侵染阶段的染色实验得知，UV-B能有效抑制病原菌侵染过程中芽管、附着胞、侵染菌丝的萌发和生长，5 kJ·m-2处理下影响程度更大，与对照相比，各阶段生长量分别减少了45.1%、82.2%和75.2%。总体来看，增强的UV-B辐射会使这些致病基因的表达下调，减少和抑制稻瘟病菌侵染阶段的生长发育，从而影响稻瘟病菌致病力，其中在5 kJ·m-2辐射强度时有更加显著的影响。 |
| 英文摘要: |
| Rice blast is a serious disease affecting rice. Four pathogenic genes in rice blast fungi were used to study the molecular pathogenicity mechanism of rice blast treated by UV-B radiation. Ultraviolet light to imitate emittances of 0, 2.5 and 5 kJ·m-2 to the rice blast and real-time PCR were used to analyze the expression of four pathogenicity-related genes (Chitinase, MGP1, MAGB, CPKA). The results showed that there was no significant change in the expression of MGP1 with an increase in the UV-B radiation dose, while the expressions of Chitinase, MAGB, and CPKA were down-regulated. After removing UV-B, expressions rebounded after 6 and 24 h. The expression of Chitinase and CPKA was greatly affected by a UV-B intensity of 5 kJ·m-2, and it was down-regulated by 94% and 61%, particularly with a radiation duration of 120 min. The change in the expression of MAGB expression was greater under 2.5 kJ·m-2, and was down-regulated by 57%, 17% and 40% from the beginning to the end of radiation. According to the dyeing experiment at the infection stage, UV-B could effectively inhibit the germination and growth of the germ tube, appressorium, and hyphae, which were 45.1%, 82.2% and 75.2% of those of the control at a radiation of 5 kJ·m-2, respectively. Overall, enhanced UV-B radiation can down-regulate the expression of these diseasecausing genes and reduce and inhibit the growth of Magnaporthe grisea in the infection stage, thus affecting its pathogenicity. Finally, the effect of a radiation intensity of 5 kJ·m-2 is more significant. |
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