文章摘要
魏帅,Wolde Tefera Beri,王润泽,林之,谢若瀚,乔亚蓓,卢玲丽,田生科.镉铜复合污染土壤中南方红豆杉的重金属提取效果及根际细菌群落特征[J].农业环境科学学报,2019,38(8):1919-1928.
镉铜复合污染土壤中南方红豆杉的重金属提取效果及根际细菌群落特征
Phytoextraction of heavy metals and characteristics of the rhizosphere bacterial community of Taxus wallichiana var. mairei on multi-metal (cadmium and copper) -contaminated soil
投稿时间:2019-03-29  
DOI:10.11654/jaes.2019-0345
中文关键词: 南方红豆杉  重金属  土壤  经济价值  细菌群落
英文关键词: Taxus wallichiana var. mairei  heavy metal  soil  economic value  bacterial community
基金项目:国家重点研发计划重点专项(2017YFD0801303);国家自然科学基金项目(41877116)
作者单位E-mail
魏帅 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
Wolde Tefera Beri 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
王润泽 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
林之 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
谢若瀚 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
乔亚蓓 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
卢玲丽 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058  
田生科 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室, 杭州 310058 tiansk@zju.edu.cn 
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中文摘要:
      为明确复合重金属污染土壤对南方红豆杉价值的影响,以高经济价值植物南方红豆杉(Taxus wallichiana var.mairei)为研究材料,在田间Cd/Cu复合污染土壤上开展大田试验,采用高通量测序和ICP-MS分析技术,研究了复合污染土壤条件下南方红豆杉根际细菌群落特征和特异细菌群落结构,测定了南方红豆杉各部位重金属的含量。结果表明:南方红豆杉的种植显著降低了根际土壤的细菌α-多样性,改变了根际土壤细菌群落结构,与非根际土壤相比,南方红豆杉的根际土壤细菌群落结构组成明显不同,南方红豆杉根际Alphaproteobacteria纲(Sphingobium、Kaistobacter)、Betaproteobacteria纲(Oxalobacteraceae、Comamonadaceae)、Gammaproteobacteria纲(Acinetobacter)和Bacteroidetes门(Sphingobacteriaceae)尤为丰富。PICRUSt分析表明南方红豆杉根际中有着丰度更高的与转运蛋白、氨基酸代谢、磷酸盐代谢相关的预测功能基因。与非根际土壤相比,南方红豆杉根际土壤的Cd、Cu含量分别显著降低18.8%和55.1%;且南方红豆杉高价值部位重金属Cd和Cu含量偏低,分别是根部的24.8%和30.7%。研究表明,南方红豆杉根际形成了特异细菌群落结构,其对于根际土壤重金属Cd、Cu有良好的萃取效果。
英文摘要:
      The economic utilization of heavy-metal-contaminated soil is an important research topic in the field of land resource utilization. We conducted a field experiment on cadmium (Cd) and copper (Cu) soil to investigate the influence of multi-contaminated conditions on the economic value of the soil. 16S rRNA gene sequencing combined with inductively coupled plasma-mass spectrometry were applied to analyze the rhizosphere bacterial community structure and concentrations of Cd/Cu in different tissues of Taxus wallichiana var. mairei under multi-metal (Cd, Cu) stress. Cultivation of T. wallichiana var. mairei significantly changed the soil bacterial diversity, in which bacterial alpha diversity was significantly reduced in the rhizosphere. There was an obvious difference in the bacterial community structure between the rhizosphere and bulk compartment. Several key taxa from the phyla Alphaproteobacteria (Sphingobium and Kaistobacter), Betaproteobacteria (Oxalobacteraceae and Comamonadaceae), Gammaproteobacteria (Acinetobacter), and Bacteroidetes (Sphingobacteriaceae) were especially abundant in the rhizosphere of T. wallichiana var. mairei. PICRUSt analysis predicted a high relative abundance of functional genes related to transporters, amino metabolism, and phosphate metabolism in the rhizosphere of T. wallichiana var. mairei. The total and available concentrations of Cd and Cu in the rhizosphere were significantly reduced by 18.8% and 55.1%, respectively, after planting T. wallichiana var. mairei. Collectively, these results demonstrate that a specific bacterial community structure is formed in the rhizosphere of T. wallichiana var. mairei, which has a beneficial effect on the phytoextraction of Cd and Cu in the rhizosphere soils.
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