2株邻苯二甲酸酯高效降解菌的筛选鉴定及其降解性能

潘琪; 孙淑; 周震峰

文章摘要

潘琪,孙淑,周震峰.2株邻苯二甲酸酯高效降解菌的筛选鉴定及其降解性能[J].农业环境科学学报,2019,38(10):2354-2361.

2株邻苯二甲酸酯高效降解菌的筛选鉴定及其降解性能

Isolation, identification, and biodegradation characteristics of two phthalic acid esters-degrading strains

投稿时间：2019-03-01

DOI：10.11654/jaes.2019-0220

中文关键词: 邻苯二甲酸酯降解菌筛选食异源物鞘氨醇菌鳗败血假单胞菌

英文关键词: phthalic acid esters isolation of degrading strain Sphingobium xenophagum Pseudomonas anguilliseptica

基金项目:国家自然科学基金项目（41201507，41301552）；青岛市民生计划项目（13-1-3-103-nsh）；山东省“两区”建设专项资金项目（2011-黄-19）

作者	单位	E-mail
潘琪	青岛农业大学资源与环境学院, 青岛 266109
孙淑	青岛农业大学资源与环境学院, 青岛 266109
周震峰	青岛农业大学资源与环境学院, 青岛 266109 青岛市农村环境工程研究中心, 青岛 266109	zfzhou@qau.edu.cn

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中文摘要:

为获得用于修复邻苯二甲酸酯（PAEs）污染的高效降解菌，通过富集培养的方法从土壤中筛选出2株PAEs降解菌（RXX-2、RXX-3），经形态观察、生化鉴定和16S rDNA序列分析对菌株进行了鉴定，并对其降解性能进行了分析。结果表明：菌株RXX-2和RXX-3初步鉴定为食异源物鞘氨醇菌（Sphingobium xenophagum）和鳗败血假单胞菌（Pseudomonas anguilliseptica）。菌株RXX-2降解PAEs的最佳条件为pH 8、温度30℃、转速175 r·min^-1、接种量1.5%；菌株RXX-3降解PAEs的最佳条件为pH 7、温度30℃、转速175 r·min^-1、接种量1.0%。在最佳降解条件下，经过5 d的培养，菌株RXX-2对邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）的降解率分别达到71.43%和52.85%，RXX-3对DBP和DEHP的降解率分别达到98.98%和62.96%，表明2株降解菌在PAEs污染环境的生物修复方面具有良好的应用前景。

英文摘要:

In this study, we used enrichment culture to screen highly efficient bacteria for remediation of environments contaminated by phthalic acid esters(PAEs). Accordingly, we detected two PAEs-degrading strains(RXX-2 and RXX-3)in soil samples. These two strains were identified on the basis of morphological, physiological, and 16S rDNA sequence phylogenetic analyses. Furthermore, the degradation performances of the two strains were investigated under different conditions. The results showed that RXX-2 and RXX-3 were strains of Sphingobium xenophagum and Pseudomonas anguilliseptica, respectively. The optimal conditions for PAEs degradation by strain RXX-2 were pH 8, a temperature of 30℃, a shaking speed of 175 r·min^-1, and a strain dosage of 1.5%, whereas the optimal conditions for PAEs degradation by strain RXX-3 were pH 7, a temperature of 30℃, a shaking speed of 175 r·min^-1, and a strain of dosage 1%. Under these optimal conditions, strain RXX-2 degraded 71.43% of di-butylphthalate(DBP) and 52.85% of di-(2-ethylhexyl) phthalate (DEHP)after 5 days, whereas RXX-3 degraded 98.98% of DBP and 62.96% of DEHP after the same period of time. Our study indicates that these two strains have potential utility as agents for the bioremediation of PAEs-contaminated environments.

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