白腐真菌对PAHs的吸附和降解特征及Tween 80和Cu<sup>2+</sup>的强化作用

班巧英; 吴东洋; 李建政; 刘琦

文章摘要

班巧英,吴东洋,李建政,刘琦.白腐真菌对PAHs的吸附和降解特征及Tween 80和Cu²⁺的强化作用[J].农业环境科学学报,2020,39(2):378-385.

白腐真菌对PAHs的吸附和降解特征及Tween 80和Cu²⁺的强化作用

Biosorption and degradation characteristics of PAHs by white rot fungi and enhancement of Tween 80 and Cu²⁺

投稿时间：2019-08-22

DOI：10.11654/jaes.2019-0930

中文关键词: 白腐真菌多环芳烃生物吸附生物降解 Tween 80 Cu²⁺

英文关键词: white rot fungi PAHs biosorption biodegradation Tween 80 Cu²⁺

基金项目:国家自然科学基金项目（51708341）

作者	单位
班巧英	山西大学环境与资源学院, 太原 030006
吴东洋	山西大学环境与资源学院, 太原 030006
李建政	哈尔滨工业大学环境学院, 哈尔滨 150090
刘琦	山西大学环境与资源学院, 太原 030006

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中文摘要:

为揭示白腐真菌对多环芳烃（PAHs）的去除特性，采用间歇培养方式考察了白腐真菌对萘、菲、芘的吸附和降解特征，以及Tween 80和Cu²⁺对白腐真菌去除萘、菲、芘的影响。吸附试验结果表明，当萘、菲、芘的初始浓度分别为30.0、1.0、0.1 mg·L^-1时，白腐真菌灭活菌对它们的吸附作用显著高于活菌，其最大吸附率分别为29.3%、38.2%、70.6%。降解试验结果表明，在培养前期，白腐真菌对萘和菲的降解能力很弱，而培养后期降解作用占总去除率的59.6%~77.3%。然而，白腐真菌对芘的累计降解率仅为28.8%。在吸附和降解的共同作用下，萘、菲、芘的去除率最终达到了46.9%、53.5%和73.7%。另外，发现Tween 80和Cu²⁺可以促进白腐真菌对PAHs的去除。当Tween 80浓度介于0.1~1.0 g·L^-1时，萘和菲的去除率随Tween 80浓度的增加而增加，而芘的去除率随Tween 80浓度的增加而呈现先增加后降低的变化趋势。Tween 80不仅可以促进白腐真菌的生长，同时能提高漆酶活性。低浓度Cu²⁺（≤ 1.25 mmol·L^-1）可以提高白腐真菌的漆酶活性，从而提高萘、菲、芘的去除率，且在Cu²⁺为0.25 mmol·L^-1条件下的强化作用最为显著，分别比对照（无Cu²⁺）提高了77.3%、60.9%和23.2%。

英文摘要:

To reveal the removal characteristics of polycyclic aromatic hydrocarbons(PAHs)by white rot fungi, the biosorption and biodegradation of naphthalene, phenanthrene, and pyrene were investigated using batch culture. The effects of Tween 80 and Cu²⁺ on the removal of naphthalene, phenanthrene, and pyrene by white rot fungi were also explored. The biosorption results showed that the biosorption of PAHs by killed white rot fungi was significantly higher than that of live fungi at the initial concentrations of naphthalene, phenanthrene, and pyrene as 30, 1.0, and 0.1 mg·L^-1, respectively. The maximum adsorption rates for naphthalene, phenanthrene, and pyrene reached 29.3%, 38.2%, and 70.6%, respectively. The biodegradation results showed that white rot fungi exhibited weak degradative ability against naphthalene and phenanthrene in the early stages, whereas degradation accounted for 59.6%~77.3% of the total removal in the later stages of cultivation. Nevertheless, the accumulative degradation rate of pyrene was 28.8%. Under the co-action of adsorption and degradation, the removal of naphthalene, phenanthrene, and pyrene was achieved to be 46.9%, 53.5%, and 73.7%, respectively. Furthermore, the present study reported that Tween 80 and Cu²⁺ were capable of facilitating the removal of PAHs. At the concentration of Tween 80 of 0.1~1.0 g· L^-1, the removal of naphthalene and phenanthrene was increased as Tween 80 concentration increased. However, pyrene removal showed a decreasing after increasing trend with increasing Tween 80 concentration. Tween 80 not only promoted the growth of white rot fungi, but also enhanced laccase activity. A low concentration of Cu²⁺(≤ 1.25 mmol·L^-1)could enhance the laccase activity of white rot fungi, thereby improving the removal of naphthalene, phenanthrene and pyrene. The strengthening effect was the most significant at 0.25 mmol·L^-1 of Cu²⁺. The removal of naphthalene, phenanthrene and pyrene was increased by 77.3%, 60.9% and 23.2%, respectively.

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