| 谷艳茹,韩秉君,黄继元,杨凤霞,张克强.天津市家庭农场养殖粪污耐药基因赋存特征及风险评估[J].农业环境科学学报,2020,39(2):394-402. |
| 天津市家庭农场养殖粪污耐药基因赋存特征及风险评估 |
| Occurrence characteristics and risk assessment of resistance genes in livestock waste from family farms in Tianjin City, China |
| 投稿时间：2019-10-24 |
| DOI：10.11654/jaes.2019-1171 |
| 中文关键词: 抗生素耐药基因 家庭农场 畜禽粪污 农田土壤 |
| 英文关键词: antibiotic resistance gene family farm livestock waste farmland soil |
| 基金项目:国家自然科学基金青年基金项目（41807399）；中国博士后科学基金项目（2018M640209）；国家重点研发计划课题（2018YF0800100） |
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| 中文摘要: |
| 为了解家庭农场养殖粪污中抗生素耐药基因（ARGs）的赋存特征及潜在风险，本研究选取天津市蓟州区22家典型的家庭农场，采用实时荧光定量PCR方法，考察了不同种类畜禽粪污中ARGs的污染特征及其对周边农田土壤的影响。结果表明，磺胺类、四环素类、喹诺酮类和大环内酯类耐药基因在家庭农场畜禽粪污中普遍存在，且四环素类耐药基因tetO、tetQ、tetW和大环内酯类耐药基因ermB含量最为丰富，同时检出了blaOXA-1、blaTEM-1和blaampC等与人类健康密切相关的β-内酰胺类抗生素耐药基因（bla基因）。此外，还发现如下规律：相比于猪场和牛场，鸡场粪污中的ARGs污染程度更为严重；在不同生育阶段的猪群中，母猪粪污中的多数耐药基因相对丰度要显著高于仔猪和育肥猪（P<0.05）；畜禽粪肥施用可显著增加土壤环境中ARGs的丰度（约8~18倍）（P<0.05）。本研究表明，家庭农场畜禽养殖粪污中耐药基因污染严重且普遍，同时随着粪污还田进入土壤环境增大环境风险，最终可能会通过污染农作物危害人类健康，应引起高度重视。 |
| 英文摘要: |
| In order to understand the occurrence characteristics and potential environmental risks of the antibiotic resistance genes(ARGs) in livestock waste from family farms, 22 typical family farms were selected to investigate the pollution characteristics of ARGs in different livestock wastes and explore the effects on surrounding farmland soils in Jizhou area of Tianjin using real-time fluorescence quantitative PCR. The results showed that the sulfonamide-, tetracyclic-, quinolone-, and macrolide-resistance genes were highly prevalent in livestock waste from these family farms. Among them, the pollution level of tetracycline resistance genes tetO, tetQ, tetW and macrolide resistance gene ermB was most severe in these family farms. In addition, β-lactam resistance gene(bla gene)that is related to human health, like blaOXA-1、blaTEM-1, and blaampC have also been detected in livestock waste. The following results were found:(1)Compared with pig waste and cattle waste, the pollution level of ARGs was more serious in chicken waste;(2)The relative abundance of most ARGs was significantly higher in sow waste than in piglet pig waste and fattening pig waste(P<0.05);(3)Application of livestock waste could significantly increase ARGs abundance in soil environment(approximately 8~18 times)(P<0.05). The above results indicate that ARGs pollution was prevalent and severe in livestock waste from family farms, which would increase the environmental risk via manure application. Finally, it may harm human health by further polluting crops; thus, ARGs contamination in family livestock farms requires more attention. |
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